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Seminal extracellular vesicles influence porcine spermatozoa physiology by modulating key functional parameters

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dc.contributor.authorParra, Ana
dc.contributor.authorMartín-Cano, Francisco E.
dc.contributor.authorMartínez Díaz, Pablo
dc.contributor.authorPanales, Patricia
dc.contributor.authorLucas Arjona, Xiomara
dc.contributor.authorRoca, Jordi
dc.contributor.authorBarranco Cascales, Isabel
dc.contributor.authorPeña, Fernando J.
dc.contributor.departmentMedicina y Cirugía Animal
dc.contributor.otherFacultad de Veterinaria
dc.date.accessioned2025-10-17T06:39:10Z
dc.date.available2025-10-17T06:39:10Z
dc.date.copyright© 2025 The Authors
dc.date.issued2025-09-27
dc.description.abstractSeminal plasma (SP) contains a heterogeneous population of extracellular vesicles (EVs) recognized as key modulators of sperm function. However, the specific functional roles of each seminal EV (sEV) subset remain poorly understood. This study aimed to evaluate the interaction of two sized sEV subsets (small [S-sEVs] and large [L-sEVs]) with pig liquid-stored spermatozoa under different pH conditions and their effect on specific sperm functional parameters. Seminal EV subsets were isolated from SP samples using size exclusion chromatography and characterized following the MISEV2023 guidelines. Semen samples were incubated with each sEV subset or without sEVs (control) for 6 h at 37 ºC, 100 % humidity and 5 % CO₂ under different pH conditions (6.5, 7.0, or 7.5). Sperm functional parameters were assessed by flow cytometry (Cytoflex®S and LX, Beckman Coulter), under capacitating and non-capacitating conditions. Confocal microscopy revealed that both sEV subsets bound to and were internalized by spermatozoa as early as 30 min after incubation, regardless of pH. Flow cytometry revealed that both sEVs decreased reactive oxygen species production (P ≤ 0.0001), mitochondrial membrane potential (P ≤ 0.0001) and mitochondrial O₂•⁻ levels (P ≤ 0.01) and increased apoptosis (active caspase-3) in viable spermatozoa (P ≤ 0.0001). However, the influence of sEV on acrosome integrity in viable sperm was time- and condition-dependent (P ≤ 0.05). This study showed that both S- and L-sEVs interact with porcine spermatozoa across a range of physiological pH conditions. This interaction is reflected by decreased oxidative stress and mitochondrial activity, as well as increased apoptosis in spermatozoa.
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dc.format.extent15
dc.identifier.citationAnimal Reproduction Science 282 (2025) 108009
dc.identifier.doihttps://doi.org/10.1016/j.anireprosci.2025.108009
dc.identifier.eissn1873-2232
dc.identifier.issn0378-4320
dc.identifier.urihttp://hdl.handle.net/10201/167329
dc.languageeng
dc.publisherElsevier
dc.relationThe study was funded by the following grants: (1) PID2020–113493RB-I00 funded by (Ministry of Science, Innovation and Universities, Spain [MICIU]) MICIU/AEI/10.13039/501100011033; (2) PID2022–137738NA-I00 funded by MICIU/AEI/10.13039/501100011033 and ERDF/EU; (3) RYC2021–034546-I funded by MICIU/AEI/10.13039/501100011033 and European Union NextGenerationEU/PRTR; and (4) 21935/PI/22 funded by Seneca Foundation (Murcia, Spain).
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0378432025002489?via%3Dihub
dc.rightsAttribution 4.0 Internacional
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectExtracellular vesicles
dc.subjectPig
dc.subjectSeminal plasma
dc.subjectSperm function
dc.subjectSpermatozoa
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleSeminal extracellular vesicles influence porcine spermatozoa physiology by modulating key functional parameters
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
relation.isAuthorOfPublication57ff06c2-c0ce-46eb-b327-b84f76532b8e
relation.isAuthorOfPublicationcce5a0d2-ce41-4215-b03d-9348fda2d53f
relation.isAuthorOfPublication3c34d0d5-7d7d-4bcc-a119-8906f53baa7b
relation.isAuthorOfPublication.latestForDiscovery57ff06c2-c0ce-46eb-b327-b84f76532b8e
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