Publication: Immunolocalizations of VEGF, its receptors flt-1, KDR and TGF-ß's in epithelial ovarian tumors
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Date
2006
Authors
Inan, S. ; Vatansever, S. ; Celik-Ozenci, C. ; Sanci, M. ; Dicle, N. ; Demir, R.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Objective: Angiogenesis is an essential factor
for growth, differentiation, invasion and metastasis of
tumors. In this study, we aimed to evaluate the
immunolocalizations of vascular endothelial growth
factor (VEGF), its receptors flt-1, KDR/flk-1, and
transforming growth factor-beta’s (TGF-ß) in epithelial
ovarian tumors, utilizing indirect immunohistochemistry
to understand the role of the angiogenic events in
ovarian neoplasia. Methods: Tissue blocks from 40
patients who had ovarian pathology (borderline
serous–mucinous tumor and malignant serous–mucinous
adenocarcinoma of the ovary) were included in this
study. All formalin-fixed, paraffin-embedded tissue
sections were stained with hematoxylin-eosin or primary
antibodies against VEGF, flt-1, KDR/flk-1, TGF-ß1,
TGF-ß2 and TGF-ß3 using the avidin-biotin-peroxidase
method. H-SCORE, a semi-quantitative grading system,
was used to compare immunohistochemical staining
intensities. Results: Positive VEGF immunoreactivity
was concentrated in the epithelial and stromal parts of all
the ovarian samples and the endothelial cells in the
stroma were also stained. Increased immunoreactivity of
VEGF was observed in malignant ovarian
adenocarcinomas compared to the borderline tumors of
the ovary. VEGF receptors, flt-1 and KDR/flk-1
immunoreactivities were detected not only in vascular
endothelial cells, but also in tumor cells at malignant
sites. Immunoreactivities of VEGF and its receptors
were coexpressed in tumor cells of the ovarian
carcinoma. While immunoreactivities of TGF-ß1 and
TGF-ß2 were both overexpressed in malignant ovarian carcinomas, immunoreactivity of TGF-ß3 was still mild.
Conclusion: Our results suggest that overexpression of
VEGF, its receptors flt-1, KDR/flk-1 and TGF-ß
interaction may play an important role in the ovarian
cancer biology, with potential effects on tumor growth
and angiogenesis. New therapeutic strategies using
VEGF and TGF-ß antagonists could obtain an additional
approach to the treatment ovarian carcinoma by
inhibiting angiogenesis.
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