Publication: Tubular changes in obstructed kidney of adult mice evaluated using immunohistochemistry for segment-specific marker
Loading...
Date
2007
Authors
Kida, Y. ; Sato, T.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The main focus of the present investigation is
to examine obstructed kidneys due to unilateral ureteral
obstruction (UUO) model in adult mice using segmentspecific
tubular marker and to confirm the detailed
morphological evaluation of UUO that is a typical model
for the tubulointerstitial fibrosis which is an endpoint
outcome of chronic renal diseases. Adult mice were
subjected to UUO, and kidneys were harvested 1, 3, 7
days after surgical operation. Expansion of interstitial
space both in the cortex and the medulla was confirmed
3 days after UUO by HE- and azan-staining. Interstitial
fibrosis developed especially around dilated tubules.
Immunohistochemistry for segment-specific antibodies
revealed that the proximal tubules and the descending
limb of Henle's loop did not dilate until 7 days after
UUO, whereas initial dilation of the ascending limb of
Henle's loop appeared to occur one day after surgery.
The segment from the distal tubules to the collecting
ducts began dilating one day after surgery and afterward
significantly dilated. The downstream segment of
nephron was involved in dilating earlier than the
upstream of nephron in obstructed kidney examined in
the present study. Moreover, the tubules accompanying
apoptosis of tubular epithelia significantly dilated
compared with those without apoptotic tubular epithelia.
From the above-mentioned findings, we conclude that
tubular dilatation of distal segment (from the ascending
limb of Henle's loop to the collecting ducts) of nephron
develops tubular epithelial apoptosis caused by
accumulated urine, which would link to tubular
disappearance and its replacement with fibrous tissue in
UUO kidney of adult mice.
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.