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Hepatic macrophage activation and the LPS pathway in patients with different degrees of severity and histopathological patterns of drug induced liver injury

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dc.contributor.authorDu, Hui-juan
dc.contributor.authorZhao, Su-xian
dc.contributor.authorZhao, Wen
dc.contributor.authorFu, Na
dc.contributor.authorLi, Wen-cong
dc.contributor.authorQin, Xiao-jie
dc.contributor.authorZhang, Yu-guo
dc.contributor.authorNan, Yue-min
dc.contributor.authorZhao, Jing-min
dc.date.accessioned2023-01-16T12:10:33Z
dc.date.available2023-01-16T12:10:33Z
dc.date.issued2021
dc.description.abstractBackground. Inflammatory activation of hepatic macrophages plays a primary role in druginduced liver injury (DILI). However, the exact mechanism underlying DILI remains unclear. Methods. A total of 328 DILI patients and 80 healthy individuals were prospectively enrolled in this study. The DILI patients were categorized into subgroups based on either disease severity or histopathological patterns. Plasma soluble CD163 (sCD163) and hepatic CD163 were examined to determine hepatic macrophage activation, and CD8, CD20, and MUM-1 were assessed to determine cellular immunity using immunohistochemistry. The lipopolysaccharide (LPS) pathway proteins [e.g. LPS, soluble CD14 (sCD14), and LPSbinding protein (LBP)] were measured using enzymelinked immunosorbent assay. Results. Plasma sCD163 levels were nine-fold higher in DILI patients than in healthy controls at the baseline, but significantly decreased at the 4-week follow-up visit after treatment. The numbers of hepatic macrophages, B cells, and plasma cells were significantly higher in the liver tissues from DILI patients than those from healthy controls. Furthermore, the baseline levels of LPS pathway proteins in the DILI patients were significantly higher than those in the controls. Notably, these proteins significantly decreased at the 4-week follow-up visit but remained significantly higher than the levels for the controls. Conclusions. Hepatic inflammation in DILI involves the activation of hepatic macrophages and cellular immunity, in which the LPS pathway likely plays a role, at least in part. As such, this study has improved our understanding of the pathological mechanisms for DILI and may facilitate the development of better treatments for patients with DILI.es
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dc.format.extent10es
dc.identifier.citationHistology and Histopathology Vol. 36, nº6 (2021)
dc.identifier.doihttps://doi.org/10.14670/HH-18-340
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/127353
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectDrug-induced liver injuryes
dc.subjectHepatic macrophageses
dc.subjectCellular immunityes
dc.subjectLPSes
dc.subjectLBPes
dc.subjectCD163es
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleHepatic macrophage activation and the LPS pathway in patients with different degrees of severity and histopathological patterns of drug induced liver injuryes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.36, nº6 (2021)