Publication: The cellular and subcellular localization of zinc transporter 7 in the mouse spinal cord
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Date
2008
Authors
Chi, Zhi-Hong ; Ren, Hao ; Wang, Xin ; Rong, Ming ; Huang, Liping ; Wang, Zhan-You
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The present work addresses the cellular and
subcellular localization of the zinc transporter 7 (ZNT7,
SLC30a7) protein and the distribution of zinc ions
(Zn2+) in the mouse spinal cord. Our results indicated
that the ZNT7 immunoreactive neurons were widely
distributed in the Rexed’s laminae of the gray matter in
all spinal segments examined. The ependyma cells of the
central canal and glia cells in the white matter were also
shown ZNT7-positive. The ZNT7 immunoreactivity was
mainly detected in the perinuclear regions of ZNT7-
positive cells in the spinal gray matter. For ependyma
cells, the immunoreactivity of ZNT7 was detected in the
cytoplasm near the lumina of the central canal.
Ultrastructural localization showed that ZNT7 was
predominately present in the membrane of the Golgi
stacks. The double immunofluorescence studies
confirmed this result. Other intracellular organelles
including the endoplasmic reticulum, mitochondria and
lysosomes were devoid of ZNT7-immunostaining. The
chelatable Zn2+ ions in the spinal cord were found
predominantly in the terminals of the neuron rather than
the cell body in the gray matter. However, overlapping distribution of chelatable Zn2+ ions and ZNT7 was found
in the ependyma cells. The present study supports the
notion that ZNT7 may function to supply zinc ions to the
newly synthesized metalloproteins in the secretory
pathway of the spinal neuron and the ependyma cell.
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