Publication: Galectin-7, will the lectinrsquos activity establish clinical correlations in head
and neck squamous cell and basal cell carcinomas?
Authors
Câda, Z. ; Chovanec, M. ; Smetana, K. Jr. ; Betka, J. ; Lacina, L. ; Plzák, J. ; Kodet, R. ; Stork, J. ; Lensch, M. ; Kaltner, H. ; André, S. ; Gabius, H.J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The human lectin galectin-7 (Gal-7; p53-
induced gene-1) has anti- and pro-malignant features in
different in vitro models. We tried to clarify relation of
its expression to cellular and clinical parameters in head
and neck squamous and basal cell carcinomas. Using a
non-cross-reactive antibody, immunohistochemical
staining in squamous cell epithelia (epidermis,
epithelium of oropharynx and larynx) (n = 57),
squamous cell carcinomas (n = 47) and lymph node
metastases (n = 25), as well as basal cell carcinomas (n =
10) were studied. This monitoring was flanked by
processing to assess the level of differentiation
(cytokeratins 10 and 14), proliferation (Ki67) and basal
lamina formation (collagen IV). The results were
correlated with clinical and pathological findings
(grading, TNM-staging, extracapsular spread, angio- and
lymphangioinvasion, perineural invasion, recurrence and
survival). Gal-7 resides in all layers of epithelia with
cytoplasmic and nuclear localization in normal
specimens. Basal cell carcinomas were devoid of the
Gal-7 respective signal. Squamous cell carcinomas were
positive, presenting different staining profiles. Intense
staining was predominantly found in squamous cell
cancers with high degrees of differentiation and
keratinization. Fittingly, poor level of differentiation (P =
0.0009), absence of keratinization (P = 0.0105) and
significant discontinuity or absence of collagen IV
expression in the peritumoral basal lamina (P = 0.0024). was found in Gal-7-negative tumors. Gal-7 presence was
not related to gender, primary tumor site, T-stage, Nstage,
clinical stage, extracapsular spread, angio- and
lymphangioinvasion, perineural spread or treatment
outcome at a statistically significant level.
Immunohistochemical analysis revealed a positive
correlation for differentiation and keratinization to Gal-7
presence in squamous cell carcinomas. Absence of Gal-7
expression was detected in basal cell carcinomas. These
clinical data delineate Gal-7 influence on differentiation
in vivo, without evidence for a role in dissemination
reported for lymphoma.
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