Publication: Expression pattern of glypican-3 -GPC3- during human embryonic and fetal development
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Date
2008
Authors
Iglesias, Bibiana V. ; Centeno, Gloria ; Pascuccelli, Hector ; Ward, Flavia ; Peters, Maria Giselle ; Puricelli, Lydia ; Bal de Kier Joffé, Elisa
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Filmus, Jorge
Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Glypicans represent a family of cell surface
proteoglycans. Loss-of-function mutations in the human
glypican-3 (GPC3) gene results in the Simpson-Golabi-
Behmel syndrome, characterized by severe
malformations and pre- and postnatal overgrowth.
Because the expression of GPC3 during human
embryonic and fetal periods remains largely unknown,
we investigated by immunohistochemistry its pattern of
expression during four periods of human development
covering the embryonic period (P1) from 5 to 8 weeks of
development, and the fetal periods (P2, P3 and P4) from
9 to 28 weeks of development.
Hepatocytes were homogeneously positive for GPC3
during the four periods while pancreatic acini and ducts
showed a rather high staining only during P1.
GPC3 was also detected in several kidney structures
and in the genital system where the sex cords were
weakly positive in P1 and P2. In later developmental
stages the male’s genital system expressed GPC3 while
the female’s did not.
While the mesenchyme in the limbs showed positive
staining in P1, GPC3 was not detected during the
following stages. The mesenchymal tissue localized
between the most caudal vertebrae was also positive in
P1.
A strong GPC3 signal was observed in neurons of
the spinal cord and dorsal root ganglia in P2 and P3,
while the brain was negative.
In sum our studies revealed that GPC3 expression is
highly tissue- and stage-specific during human
development. The expression pattern of GPC3 is consistent with the abnormalities seen in the Simpson-
Golabi-Behmel syndrome.
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