Publication:
Different expression of protein kinase A (PKA) regulatory subunits in normal and neoplastic thyroid tissues

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Date
2015
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Authors
Ferrero, Stefano ; Vaira, Valentina ; Del Gobbo, Alessandro ; Vicentini, Leonardo ; Bosari, Silvano ; Beck-Peccoz, Paolo ; Mantovan, Giovanna ; Spada, Anna ; Lania, Andrea G.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
http://doi.org/10.14670/HH-30.473
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info:eu-repo/semantics/article
Description
Abstract
The four regulatory subunits (R1A, R1B, R2A, R2B) of protein kinase A (PKA) are differentially expressed in several cancer cell lines and exert distinct roles in both cell growth and cell differentiation control. Mutations of the PRKAR1A gene have been found in patients with Carney complex and in a minority of sporadic anaplastic thyroid carcinomas. The aim of the study was to retrospectively evaluate the expression of different PKA regulatory subunits in benign and non benign human thyroid tumours and to correlate their expression with clinical phenotype. Immunohistochemistry demonstrated a significant increase in PRKAR2B expression in both differentiated and undifferentiated (anaplastic) thyroid tumors in comparison with normal thyroid tissues. Conversely, a significant increase in PRKAR1A expression was only demonstrated in undifferentiated thyroid carcinomas in comparison with normal thyroid tissue and differentiated thyroid tumors. In thyroid cancers without lymph nodal metastases PRKAR1A expression was higher in tumours of more than 2 cm in size (T2 and T3) compared to smaller ones (T1). In conclusion, our data shows that an increased PRKAR1A expression is associated with aggressive and undifferentiated thyroid tumors.
Citation
Histology and Histopathology, Vol. 30, n.º 4 (2015)
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