Publication: Efficacy of expressions of Arg-1, Hep Par-1, and CK19 in the diagnosis of the primary hepatocellular carcinoma subtypes and exclusion of the metastases
Authors
Atta, Ihab Shafek
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-361
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info:eu-repo/semantics/article
Description
Abstract
Many conflicts arise using immunohistochemistry of Hepatocellular carcinoma (HCC),
some of these conflicts arise from the biliary part within
the tumor itself or from liver metastasis. The aim of this
study is to investigate the extent of Arg-1, HepPar-1, and
CK-19 expressions in the primary HCC subtypes as well
as studying of some metastatic cases to find a distinctive
immunohistochemical panel utilizing it to differentiate
between these entities.
Material and methods. A paraffin-embedded block
including 62 cases of primary HCC, and 18 cases
diagnosed as metastatic tumors, were subjected for this
study using Anti-liver Arginase antibody (ab125134
Cambridge, USA, polyclonal antibody, 3.75 µg/ml),
HepPar-1 (polyclonal mouse antibody OCH1E5; 1:600;
DAKO, CA, USA), and CK 19 Anti-Cytokeratin 19
antibody (ab15463, rabbit polyclonal antibody; 1:100;
Cambridge, USA). The intensity of immunostaining was
scored (0 to 3+). Nuclear and cytoplasmic staining with
Arg-1 and cytoplasmic for both HepPar-1 and CK 19 are
reported.
Results. The histopathological patterns were mainly
trabecular no=(24, 38.7%), and pseudoglandular (no=14,
22.5%), mixed hepatocellular cholangio-carcinoma was
observed in one case (1.6%). Arginase-1 positivity was
in 55 cases (88.7%) opposite to 46 (74.19%) and 8
(12.9%) for HepPAr.1% -1 and CK 19, respectively. The
intensity of expression was marked in well and moderate
differentiation for Arg-1 and HepPar-1and in poorly
differentiated for CK 19. Metastatic carcinoma cases
revealed two cases positive for Arg-1 (11.1%), 4 cases
(22.2%) positive for HepPar-1, and 13 cases (72.2%)
positive for CK 19.
Conclusion. Arg-1 and HepPar-1 are confirmative in
the diagnosis of primary HCC in most cases, either
separately or collectively but the priority of selection
leans more towards Arg-1. Arg-1 and HepPar-1 positive
with negative CK 19 expressions give more support to
diagnosis of primary HCC while the reverse will support
the diagnosis of tumour of biliary origin or liver
metastasis.
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Citation
Histology and Histopathology Vol. 36, nº9 (2021)
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