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Impact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance

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dc.contributor.authorCatalán, Jaime
dc.contributor.authorYáñez Ortiz, Iván
dc.contributor.authorTvarijonaviciute, Asta
dc.contributor.authorGonzález Arostegui, Luis Guillermo
dc.contributor.authorPeres Rubio, Camila
dc.contributor.authorYeste, Marc
dc.contributor.authorMiró, Jordi
dc.contributor.authorBarranco Cascales, Isabel
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2025-10-17T08:29:21Z
dc.date.available2025-10-17T08:29:21Z
dc.date.copyright© 2022 by the authors
dc.date.issued2022-02-18
dc.description.abstractThis study investigated whether the activities of the antioxidant components of donkey seminal plasma (SP)-both enzymatic (superoxide dismutase (SOD), catalase-like (CAT), glutathione peroxidase-like (GPX), and paraoxonase type 1 (PON1)) and non-enzymatic (measured in terms of total thiol, copper-reducing antioxidant capacity (CUPRAC), ferric-reducing ability of plasma (FRAP), and Trolox equivalent antioxidant capacity (TEAC))-and oxidative stress index (OSI) are related to sperm cryotolerance. For this purpose, 15 ejaculates from jackasses (one per individual) were collected and split into two aliquots. The first one was used for measuring the activities levels of enzymatic and non-enzymatic antioxidants and OSI in SP, whereas the other aliquot was cryopreserved. Before cryopreservation, sperm quality parameters (concentration, motility, and viability) were evaluated. After thawing, sperm motility, plasma membrane integrity, lipid disorder, mitochondrial membrane potential, reactive oxygen species (ROS), and calcium intracellular levels were also determined. Based on the percentages of total motility (TM) and of sperm with an intact plasma membrane (SYBR14+/PI-) after thawing, samples were classified as good-freezability (GFE) or poor-freezability (PFE) ejaculates through cluster analyses. The SP activity levels of enzymatic (SOD and PON1) and non-enzymatic antioxidants (CUPRAC, FRAP, and TEAC) were higher (p < 0.05) in GFE than in PFE, whereas SP-OSI was higher (p < 0.05) in PFE than in GFE. In addition, the activity levels of SOD, PON1, GPX, CUPRAC, FRAP, and TEAC were positively (p < 0.05) related to post-thaw sperm motility and plasma membrane integrity and negatively to intracellular ROS levels. The SP-OSI was negatively correlated (p < 0.05) to post-thaw sperm quality parameters and positively to intracellular ROS levels. It can thus be concluded that donkey SP antioxidants are related to sperm cryotolerance and that measurements of antioxidants PON1, SOD, CUPRAC, FRAP, and TEAC, as well as SP-OSI, could be used as markers of sperm cryotolerance. Further research addressing the relationship of these antioxidants and SP-OSI with sperm cryotolerance and their potential use as freezing markers is warranted.
dc.formatapplication/pdf
dc.format.extent22
dc.identifier.citationAntioxidants, 2022, Vol. 11, Issue 2 : 417
dc.identifier.doihttps://doi.org/10.3390/antiox11020417
dc.identifier.eissn2076-3921
dc.identifier.urihttp://hdl.handle.net/10201/167371
dc.languageeng
dc.publisherMDPI
dc.relationJ.C. was funded by the project “Demetra” (Dipartamente di Eccellenza 2018–2022, CUP_C46C18000530001), Italian Ministry for Education, University and Research. I.Y.-O. was funded by the Secretary of Higher Education, Science, Technology and Innovation (SENESCYT), Ecuador (Scheme: Programa de Becas Internacionales de Posgrado 2019; Grant: CZ02-000507-2019). The authors also acknowledge the support from the Ministry of Science and Innovation, Spain (Grant: AGL2017-88329-R), and the Regional Government of Catalonia (2017-SGR-1229). I.B. was financially supported by European Union’s Horizon 2020 research and innovation program (H2020-MSCA-IF-2019-891382). L.G-A. was funded by Fundación Séneca, Región de Murcia, Spain (21453/FPI/20). C.P-R. has a post-doctoral fellowship “Juan de la Cierva Formación” supported by the “Ministerio de Economía y Competitividad” (FJC2019-042475-I), Spain.
dc.relation.publisherversionhttps://www.mdpi.com/2076-3921/11/2/417
dc.rightsAttribution 4.0 International
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAntioxidants
dc.subjectCryopreservation
dc.subjectDonkey
dc.subjectReactive oxygen species
dc.subjectROS
dc.subjectSeminal plasma
dc.subjectSP
dc.subjectSperm
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleImpact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
relation.isAuthorOfPublication51159b46-4514-4a54-9cc7-dea538fc1efa
relation.isAuthorOfPublication87fbaa15-1b64-4b43-8aea-a3384f66f772
relation.isAuthorOfPublication3c34d0d5-7d7d-4bcc-a119-8906f53baa7b
relation.isAuthorOfPublication.latestForDiscovery51159b46-4514-4a54-9cc7-dea538fc1efa
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