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Sensitivity of two methods to detect Mycoplasma agalactiae in goat milk

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dc.contributor.advisorAmores, Joaquín
dc.contributor.authorTatay-Dualde, Juan
dc.contributor.authorPrats- Van der Ham, Miranda
dc.contributor.authorGómez-Martín, Angel
dc.contributor.authorPaterna, Ana
dc.contributor.authorCorrales, Juan Carlos
dc.contributor.authorSánchez, Antonio
dc.contributor.authorFe, Christian de la
dc.contributor.authorAmores, Joaquín
dc.contributor.departmentSanidad Animal
dc.coverage.temporal2015es
dc.date.accessioned2024-02-12T07:56:12Z
dc.date.available2024-02-12T07:56:12Z
dc.date.created2015
dc.date.issued2015
dc.description© 2015 Tatay-Dualde et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.description.abstractBackground: Laboratory diagnostic techniques able to detect Mycoplasma agalactiae are essential in contagious agalactia in dairy goats. This study was designed: 1) to determine the detection limits of PCR and culture in goat milk samples, 2) to examine the effects of experimental conditions including the DNA extraction method, PCR technique and storage conditions (fresh versus frozen stored milk samples) on these methods and 3), to establish agreement between PCR and culture techniques using milk samples from goats with mastitis in commercial dairy herds. The study was conducted both on artificially inoculated and field samples. Results: Our findings indicate that culture is able to detect M. agalactiae in goat milk at lower concentrations than PCR. Qualitative detection of M.agalactiae by culture and PCR was not affected by sample freezing, though the DNA extraction method used significantly affected the results of the different PCR protocols. When clinical samples were used, both techniques showed good agreement. Conclusions: The results from this study indicate that both culture and PCR are able to detect M. agalactiae in clinical goat mastitis samples. However, in bulk tank milk samples with presumably lower M. agalactiae concentrations, culture is recommended within the first 24 h of sample collection due to its lower limit of detection. To improve the diagnostic sensitivity of PCR in milk samples, there is a need to increase the efficiency of extracting DNA from milk samples using protocols including a previous step of enzymatic digestion.es
dc.formatapplication/pdfes
dc.format.extent4es
dc.identifier.doi10.1186/s13620-015-0049-y
dc.identifier.eisbnIrish Veterinary Journal 2015 68:21es
dc.identifier.issn2046-0481
dc.identifier.urihttp://hdl.handle.net/10201/139259
dc.languageenges
dc.publisherBioMed Centrales
dc.relationFinanciación: Plan Nacional de I + D+I Del Ministerio de Economía y Competitividad. España. AGL2013-44771-R. VARIABILIDAD GENÓMICA DE MICOPLASMAS ASOCIADOS A LA AGALAXIA CONTAGIOSA Y SU RELACIÓN CON LA DINÁMICA DE LA INFECCIÓN Y LA RESISTENCIA ANTIBIÓTICA. Proyecto Nacionales
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectMycoplasmaes
dc.subjectSensibilidades
dc.subjectGoaten
dc.subjectContagious agalactiaen
dc.subjectPCRen
dc.subjectCultureen
dc.subjectMilken
dc.subjectMycoplasma agalactiaeen
dc.subject.otherCDU::5 - Ciencias puras y naturaleses
dc.titleSensitivity of two methods to detect Mycoplasma agalactiae in goat milkes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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