Publication: Fate and functions of human adult lymphoid cells in immunodeficient mice
Authors
Vallet, V. ; Cherpillod, J. ; Waridel, F. ; Duchosal, M.A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Laboratory models enabling to study in vivo
human leukocyte functions have been developed. Most
of the models consist of human immunocytes transferred
to mice homozygous for the scid mutation. Mice with
additional immunodeficient-prone genetic background or
with immunodeficiency-induced conditioning have also
been used. Human grafts mainly consisted of human
immune cells in suspension injected intraperitoneally, or
in pieces of human organs containing immunocytes
implanted subcutaneously. Cells in suspension could be
easily manipulated in vitro before transfer to the animal,
but disseminated within the mouse body. In opposition,
human cells mostly remained within implantation areas
of animals given human organ pieces. This favorizes cell
interactions and helps for cell recovery after their in vivo
passage. Moreover, the diversity of antibodies in animals
transplanted with human lymphoid organ pieces
appeared broader than that of mice transferred with
lymphocytes in suspension. Spontaneous recall antibody
and autoantibody productions have been generally
observed in animals transferred with cells from donors
with such antibodies. In vivo boosting of recall antibody
by antigen has been most successful, but such a
manipulation inconstantly boosted autoantibodies.
Primary human T and B cell responses were difficult to
obtain in xenochimeric animals, and success has been
generally obtained by optimizing human immune
response parameters, such as antigen presentation.
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