Analytical validation of commercially available methods for acute phase proteins quantification in pigs

Tecles Vicente, Fernando; Fuentes, P.; Martínez Subiela, Silvia; Parra Muñoz, María Dolores; Muñoz, A.; Cerón Madrigal, José Joaquín

Publication:
Analytical validation of commercially available methods for acute phase proteins quantification in pigs

dc.contributor.author	Tecles Vicente, Fernando
dc.contributor.author	Fuentes, P.
dc.contributor.author	Martínez Subiela, Silvia
dc.contributor.author	Parra Muñoz, María Dolores
dc.contributor.author	Muñoz, A.
dc.contributor.author	Cerón Madrigal, José Joaquín
dc.contributor.department	Ciencias Sociosanitarias
dc.date.accessioned	2025-01-21T11:45:39Z
dc.date.available	2025-01-21T11:45:39Z
dc.date.issued	2006-12-01
dc.description	© 2006 Elsevier Ltd. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Research in Veterinary Science. To access the final edited and published work see https://doi.org/ 10.1016/j.rvsc.2006.10.005	es
dc.description.abstract	The aim of this study was to validate commercially available methods for porcine haptoglobin (Hp), C-reactive protein (CRP), serumamyloid A (SAA) and major acute phase protein (Pig-MAP) determinations. Intra and inter assay coefficients of variation (CVs) were lower than 20% in all cases with exception of inter assay CVs for CRP and Pig-MAP assays with samples of low acute phase proteins concentration, and for SAA assay at any acute phase proteins concentration. All methods showed good linearity and detection limits were low enough to detect APPs levels in healthy animals. Hp and SAA were very affected by haemolysis. Lipaemia influenced mainly on SAA determination. Over 15-fold increase was observed in CRP and SAA concentrations after artificially induced inflammation by a single subcutaneous dose of turpentine, whereas Hp and Pig-MAP increased less than 5-fold.	es
dc.format	application/pdf	es
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dc.identifier.citation	Research in Veterinary Science 83 (2007) 133–139
dc.identifier.doi	https://doi.org/10.1016/j.rvsc.2006.10.005
dc.identifier.issn	Print: 0034-5288
dc.identifier.issn	Electronic: 1532-2661
dc.identifier.uri	http://hdl.handle.net/10201/148905
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	Support from the Seneca Foundation of Murcia (PB/13/FS/02) made this study possible.	es
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dc.rights	info:eu-repo/semantics/embargoedAccess	es
dc.subject	Acute phase proteins	es
dc.subject	Porcine	es
dc.subject	Validation	es
dc.subject	Haptoglobin	es
dc.subject	C-reactive protein	es
dc.subject	Serum amyloid A	es
dc.subject	Porcine major acute phase protein	es
dc.title	Analytical validation of commercially available methods for acute phase proteins quantification in pigs	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es