Publication: Quantitative phase microscopy for evaluation of intestinal inflammation and wound healing utilizing label-free biophysical markers
Authors
Bettenworth, Dominik ; Bokemeyer, Arne ; Poremba, Christopher ; Ding, Nik Sheng ; Ketelhut, Steffi ; Lenz, Philipp ; Kemper, Björn
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-937
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info:eu-repo/semantics/article
Description
Abstract
Inflammatory bowel diseases (IBD) are
inflammatory disorders of the gastrointestinal tract
characterized by a chronic relapsing disease course. As
uncontrolled intestinal inflammation can result in severe
disease complications, recent treatment targets of IBD
evolved toward seeking the absence of mucosal and
histological inflammation. However, this approach
requires adequate histological evaluation of IBD disease
activity. The diagnostic challenge of histological
examination of intestinal inflammation is documented by
the multitude of proposed histological scoring systems.
In this context, we review quantitative phase imaging
(QPI) techniques such as digital holographic microscopy
(DHM) for characterizing intestinal inflammation. DHM
determines optical path-length delays in a stain-free
manner, thereby providing the tissue refractive index as
a biophysical marker that directly correlates to tissue
density. Recently, DHM has been successfully applied in
cell biology, cancer cell research and infectious-induced
cellular alterations. We summarized the capabilities of
DHM and related QPI techniques to assess the severity
of intestinal inflammation in experimental colitis as well
as in colonic samples from human IBD patients.
Moreover, we illustrate major advantages of DHM
facilitated multimodal evaluation of epithelial wound
healing processes as assessed by physical parameters
like cell volume, density, thickness and dry mass in
vitro. Furthermore, potential limitations of DHM and
future utilities of QPI are discussed. In conclusion, DHM
represents a promising, easy-to-use quantitative tool to
provide accurate and objective assessment of intestinal
inflammation and may pave the way towards automated
label-free digital pathology and related in vitro cell
culture analysis in future.
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Citation
Histology and Histopathology, Vol.33, nº5, (2018)
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