Publication:
TNF-α-driven inflammation and mitochondrial dysfunction define the platelet hyperreactivity of aging.

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Date
2019-08-29
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Authors
Davizon-Castillo, Pavel ; McMahon, Brandon ; Águila Martínez, Sonia ; Bark, David ; Ashworth, Katrina ; Allawzi, Ayed ; Campbell, Robert A. ; Montenont, Emilie ; Nemkov, Travis ; D’Alessandro, Angelo ; Clendenen, Nathan ; Shih, Lauren ; Sanders, Natalie A. ; Higa, Kelly ; Cox, Allaura ; Padilla-Romo, Zavelia ; Hernandez, Giovanni ; Wartchow, Eric ; Trahan, George D. ; Nozik-Grayck, Eva ; Jones, Kenneth ; Pietras, Eric M. ; DeGregori, James ; Rondina, Matthew T. ; Di Paola, Jorge
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Publisher
American Society of Hematology (ASH Publications)
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Medicina Interna
DOI
https://doi.org/10.1182/blood.2019000200
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Abstract
Aging and chronic inflammation are independent risk factors for the development of atherothrombosis and cardiovascular disease. We hypothesized that aging- associated inflammation promotes the development of platelet hyperreactivity and increases thrombotic risk during aging. Functional platelet studies in aged-frail adults and old mice demonstrated that their platelets are hyperreactive and form larger thrombi.Weidentified tumor necrosis factor a (TNF-a) as the key aging-associated proinflammatory cytokine responsible for platelet hyperreactivity. We further showed that platelet hyperreactivity is neutralized by abrogating signaling through TNF-a receptors in vivo in a mouse model of aging. Analysis of the bone marrow compartments showed significant platelet-biased hematopoiesis in old mice reflected by increased megakaryocyte-committed progenitor cells, megakaryocyte ploidy status, and thrombocytosis. Single-cell RNA-sequencing analysis of native mouse megakaryocytes showed significant reprogramming of inflammatory, metabolic, and mitochondrial gene pathways in old mice that appeared to play a significant role in determining platelet hyperreactivity. Platelets from old mice (where TNF-a was endogenously increased) and from young mice exposed to exogenous TNF-a exhibited significant mitochondrial changes characterized by elevated mitochondrial mass and increased oxygen consumption during activation. These mitochondrial changes were mitigated upon TNF-a blockade. Similar increases in platelet mitochondrial mass were seen in platelets from patients with myeloproliferative neoplasms, where TNF-a levels are also increased. Furthermore, metabolomics studies of platelets from young and old mice demonstrated agedependent metabolic profiles that may differentially poise platelets for activation. Altogether, we present previously unrecognized evidence that TNF-a critically regulates megakaryocytes resident in the bone marrow niche and agingassociated platelet hyperreactivity and thrombosis. (Blood. 2019;134(9):727-740)
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Citation
Blood® 29 August 2019 | Volume 134, Number 9, 727-740
URI
http://hdl.handle.net/10201/205301
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