Publication:
Ex vivo detection of lipopolysaccharide immunopositivity in Rushton bodies

Simple item page
dc.contributor.authorVirkkunen, Sirke
dc.contributor.authorWillberg, Jaana
dc.contributor.authorHaglund, Caj
dc.contributor.authorSund, Malin
dc.contributor.authorSorsa, Timo
dc.contributor.authorHagström, Jaana
dc.date.accessioned2023-09-07T10:29:35Z
dc.date.available2023-09-07T10:29:35Z
dc.date.issued2023
dc.description.abstractAim. Our aim was to investigate how bacterial lipopolysaccharide (LPS) is immunoexpressed in periapical lesions. By surprise we detected Rushton bodies (RBs) whose origin has been debatable to be positive for LPS. Methodology. Samples of radicular cysts (N=70) were stained in order to identify variations in LPS immunoexpression indicating bacterial background. For immunostaining, we used an anti-LPS antibody from Escherichia coli, and for visualization Horse Radish Peroxidase labeled polymer as the secondary antibody. Results. RBs showed positivity for LPS in radicular cysts. After collection of radicular cyst samples (70 in total), we noted that all RBs (N=25) histologically detected in tissue samples were positive for LPS. Furthermore, calcification in the cyst capsule showed immunopositivity. Conclusion. We demonstrate for the first time that LPS is present in RBs, indicating that host response to bacteria might be the initial cause of the formation of these hyaline bodies in the cyst epithelium and cyst capsule calcifications.es
dc.formatapplication/pdfes
dc.format.extent4es
dc.identifier.citationHistology and Histopathology Vol. 38, nº8 (2023)
dc.identifier.doihttps://doi.org/10.14670/HH-18-602
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/133730
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectLipopolysaccharidees
dc.subjectPeriapical lesionses
dc.subjectRadicular cystes
dc.subjectRushton bodieses
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleEx vivo detection of lipopolysaccharide immunopositivity in Rushton bodieses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
4b03ce3b-3b54-476e-9bb9-53a85c7b27d7.pdf
Size:
3.1 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.26 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Histology and histopathology Vol.38, nº8 (2023)