Publication: Treatment with tacrolimus enhances alveolar bone formation and decreases osteoclast number in the maxillae: A histomorphometric and ultrastructural study in rats
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Date
2008
Authors
Andia, Denise Carleto ; Nassar, Carlos Augusto ; Oehlmeyer Nassar, Patricia ; Rodrigues Guimarães, Morgana ; Cerri, Paulo Sérgio ; Spolidorio, Luis Carlos
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Recent studies have suggested that
tacrolimus monotherapy is a beneficial therapeutic
alternative for the normalization of cyclosporin-induced
bone loss in animal models and humans. The mechanism
accounting for this action is unclear at present. In the
present study, we attempted to determine the effect of
tacrolimus monotherapy on alveolar bone using
histological, histomorphometrical and transmission
electron microscopy (TEM).
Groups of rats (n=10 each) were treated with either
tacrolimus (1mg/kg/day, s.c.) or drug vehicle for 60
days. Fragments containing maxillary molars were
processed for light microscopy to investigate the
alveolar bone volume, trabecular separation, number of
osteoclasts and osteoblasts, and transmission electron
microscopy to investigate their ultrastructural basic
phenotype.
Treatment with tacrolimus monotherapy during 60
days may induce increases in alveolar bone volume
(BV/TV,%; P<0.05) and a non-significant decrease in
trabecular separation (Tb.Sp,mm; P>0.05), represented
by a decrease in osteoclast number (N.Oc/BS; P<0.05)
and maintenance of osteoblast number (N.Ob/BS;
P>0.05). Osteoblasts were often observed as a
continuous layer of active cells on the bone surface.
Osteoclasts appeared to be detached from the resorbed
bone surface, which was often filled by active
osteoblasts and collagen-rich matrix. Moreover,
osteoclasts in the treated group were frequently observed as inactive cells (without ruffled border, clear zone and
detached from the bone surface).
Within the limits of the present study, we conclude
that tacrolimus leads to an increase in alveolar bone
formation, which probably exerts action on osteoclasts.
Tacrolimus could, therefore, play a crucial role in the
control of both early osteoclast differentiations from
precursors, as well as in functional activation.
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