Publication: The long-term effects of rapamycin-based
immunosuppressive protocols on the expression of
renal aquaporins 1, 2, 3 and 4 water channels in rats
Authors
Grabowska, Marta ; Michałek, Katarzyna ; Kędzierska-Kapuza, Karolina ; Kram, Andrzej ; Gill, Kamil ; Piasecka, Małgorzata
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-321
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info:eu-repo/semantics/article
Description
Abstract
Background. To this day, the effect of multidrug immunosuppressive protocols on renal expression
of AQPs is unknown. This study aimed to determine the
influence of rapamycin-based multi-drug immunosuppressive regimens on the expression of aquaporins
(AQPs) 1, 2, 3, and 4 in the rat kidney.
Methods. For 6 months, 24 male Wistar rats were
administered immunosuppressants, according to the
three-drug protocols used in patients after organ
transplantation. The rats were divided into four groups:
the control group, the TRP group (tacrolimus,
rapamycin, prednisone), the CRP group (cyclosporine A,
rapamycin, prednisone), and the MRP group
(mycophenolate mofetil, rapamycin, prednisone).
Selected red cell indices and total calcium were
measured in the blood of rats and quantitative analysis of
AQP1, AQP2, AQP3 and AQP4 immunoexpression in
the kidneys were performed.
Results. In the TRP and CRP groups, a mild increase
of mean corpuscular hemoglobin concentration,
hematocrit and total calcium were observed. Moreover,
decreased expression of AQP1-4 was found in all
experimental groups, with the highest decrease in the
CRP group.
Conclusions. The long-term immunosuppressive
treatment using multi-drug protocols decreased AQP1-4
expressions in renal tubules, possibly leading to
impaired urine-concentrating ability in rat
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Citation
Histology and Histopathology Vol. 36, nº4 (2021)
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