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RFWD3 acts as a tumor promotor in the development and progression of bladder cancer

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dc.contributor.authorJiang, Peng
dc.contributor.authorXu, Zhijie
dc.contributor.authorWu, Shan
dc.contributor.authorSun, Junjie
dc.contributor.authorTian, Junjie
dc.date.accessioned2023-09-11T07:41:40Z
dc.date.available2023-09-11T07:41:40Z
dc.date.issued2023
dc.description.abstractBackground. Bladder cancer is one of the most commonly diagnosed malignancies of the urinary system with relatively poor prognosis and insufficient treatment strategies. RFWD3 is an E3 ligase whose function is rarely investigated in malignant tumors. Methods. A tissue microarray was used for evaluating RFWD3 expression in clinical samples and its correlation with tumor characteristics and patients’ prognosis. RFWD3 knockdown and overexpression cell models were constructed for conducting loss-of-function and gain-of-function assays. qPCR and western blotting were used for detecting mRNA and protein levels of RFWD3, respectively. MTT assay, colony formation assay, flow cytometry, wound-healing assay and transwell assay were carried out to demonstrate the change of cell phenotypes upon RFWD3 knockdown. Results. RFWD3 expression was relatively higher in bladder cancer tissues than in normal tissues, which is correlated with higher N stage and poorer prognosis of patients. Knockdown of RFWD3 in bladder cancer cells significantly inhibited cell proliferation, colony formation, promote cell apoptosis and restrained cell migration. Overexpression of RFWD3 induced the opposite effects. Conclusions. It was illustrated that RFWD3 possesses excellent tumor-promoting ability in bladder cancer. Accordingly, RFWD3 may be a promising therapeutic target in the targeted therapy of bladder cancer, which is worth further research.es
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dc.format.extent12es
dc.identifier.citationHistology and Histopathology Vol. 38, nÂş8 (2023)
dc.identifier.doihttps://doi.org/10.14670/HH-18-558
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/133803
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiaciĂłn externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBladder canceres
dc.subjectRFWD3es
dc.subjectPhenotypeses
dc.subjectTumor promoteres
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - PatologĂ­a. Medicina clĂ­nica. OncologĂ­a::616.1 - PatologĂ­a del sistema circulatorio, de los vasos sanguĂ­neos. Transtornos cardiovasculareses
dc.titleRFWD3 acts as a tumor promotor in the development and progression of bladder canceres
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.38, nÂş8 (2023)