Publication:
Role of skeletal muscle in ear development

Simple item page
dc.contributor.authorRot, Irena
dc.contributor.authorBaguma Nibasheka, Mark
dc.contributor.authorCostain, Willard J.
dc.contributor.authorHong, Paul
dc.contributor.authorTafra, Robert
dc.contributor.authorMardesic Brakus, Snjezana
dc.contributor.authorMrduljas Djujic, Natasa
dc.contributor.authorSaraga Babic, Mirna
dc.contributor.authorKablar, Boris
dc.date.accessioned2022-03-07T12:26:48Z
dc.date.available2022-03-07T12:26:48Z
dc.date.issued2017
dc.description.abstractThe current paper is a continuation of our work described in Rot and Kablar, 2010. Here, we show lists of 10 up- and 87 down-regulated genes obtained by a cDNA microarray analysis that compared developing Myf5-/-:Myod-/- (and Mrf4-/-) petrous part of the temporal bone, containing middle and inner ear, to the control, at embryonic day 18.5. Myf5-/-:Myod-/- fetuses entirely lack skeletal myoblasts and muscles. They are unable to move their head, which interferes with the perception of angular acceleration. Previously, we showed that the inner ear areas most affected in Myf5-/- :Myod-/- fetuses were the vestibular cristae ampullaris, sensitive to angular acceleration. Our finding that the type I hair cells were absent in the mutants’ cristae was further used here to identify a profile of genes specific to the lacking cell type. Microarrays followed by a detailed consultation of web-accessible mouse databases allowed us to identify 6 candidate genes with a possible role in the development of the inner ear sensory organs: Actc1, Pgam2, Ldb3, Eno3, Hspb7 and Smpx. Additionally, we searched for human homologues of the candidate genes since a number of syndromes in humans have associated inner ear abnormalities. Mutations in one of our candidate genes, Smpx, have been reported as the cause of X-linked deafness in humans. Our current study suggests an epigenetic role that mechanical, and potentially other, stimuli originating from muscle, play in organogenesis, and offers an approach to finding novel genes responsible for altered inner ear phenotypes.es
dc.formatapplication/pdfes
dc.format.extent14es
dc.identifier.citationHistology and Histopathology, Vol.32, nº10, (2017)
dc.identifier.doiDOI: 10.14670/HH-11-886
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/117701
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectMouse embryoes
dc.subjectInner eares
dc.subjectCrista ampullarises
dc.subjectType I hair celles
dc.subjectMicroarrayes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleRole of skeletal muscle in ear developmentes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Rot-32-987-1000-2017.pdf
Size:
1.57 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.39 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Histology and histopathology Vol.32,nº10 (2017)