Publication: Passive Ca(2+) overload in H9c2 cardiac myoblasts: assessment of cellular damage and cytosolic Ca(2+) transients
Authors
Lax Pérez, Antonio Manuel ; Asensio López, María del Carmen ; Pascual Figal, Domingo Andrés ; Fernandez Belda, Francisco ; Soler Pardo, Fernando
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.abb.2011.05.019
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info:eu-repo/semantics/article
Description
© 2011 Elsevier Inc. This document is the Published version of a Published Work that appeared in final form in Archives of Biochemistry and Biophysics. To access the final edited and published work see https://doi.org/10.1016/j.abb.2011.05.019
Abstract
Increase of resting Ca(2+) levels and amplitude of vasopressin-induced Ca(2+) transients were observed when cells in serum-free medium were exposed to 5mM Ca(2+) for 2h. Small effect on cell viability was also observed. A rapid cytotoxic effect was developed in the presence of 10mM Ca(2+) and absence of serum. However, cells exposed to 10mM Ca(2+) in the presence of serum were protected from damage for at least 2days. Resting Ca(2+) levels and cytosolic Ca(2+) transients in serum-containing medium with 10mM Ca(2+) displayed lower increases and a tendency to recover control values. When serum was absent, cells preincubated with 10mM Ca(2+) were more sensitive to thapsigargin-induced damage than cells preincubated with lower Ca(2+). The sensitivity was similar when serum was present. Tolerance to high Ca(2+) in the presence of serum was linked to potentiation of the mitochondrial Ca(2+) entry to decrease the sarcoplasmic reticulum Ca(2+) overload.
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Citation
Archives of Biochemistry and Biophysics, 2011, Vol. 512, Issue 2, pp. 175-182
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