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Performance evaluation of nine reference centers and comparison of DNA extraction protocols for effective surveillance of Leishmania-infected Phlebotomine sand flies: basis for technical recommendations

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dc.contributor.authorPrudhomme, Jorian
dc.contributor.authorAlten, Bulent
dc.contributor.authorDelabarre, Aymeric
dc.contributor.authorBerberoglu, Umut
dc.contributor.authorBerriatua Fernández de Larrea, Eduardo
dc.contributor.authorBongiorno, Gioia
dc.contributor.authorCristovao, José Manuel
dc.contributor.authorDavidovich Cohen, Maya
dc.contributor.authorDi Muccio, Trentina
dc.contributor.authorKasap, Ozge Erisoz
dc.contributor.authorFlorentino, Eleonora
dc.contributor.authorKirstein, Oscar D.
dc.contributor.authorKniha, Edwin
dc.contributor.authorMaia, Carla
dc.contributor.authorMungan, Mesut
dc.contributor.authorMuñoz Hernández, Clara
dc.contributor.authorNalçaci, Muhammed
dc.contributor.authorOguz Kaskan, Gizem
dc.contributor.authorOzbel, Yusuf
dc.contributor.authorOzensoy Toz, Seray
dc.contributor.authorParreira, Ricardo
dc.contributor.authorPlatzgummer, Katharina
dc.contributor.authorPolat, Ceylan
dc.contributor.authorRisueño Iranzo, José
dc.contributor.authorStudentsky, Liora
dc.contributor.authorVarol, Gamze
dc.contributor.authorWalochnik, Julia
dc.contributor.authorYetişmiş, Kardelen
dc.contributor.authorRobert-Gangneux, Florence
dc.contributor.departmentSin departamento asociado
dc.date.accessioned2026-01-19T13:30:16Z
dc.date.available2026-01-19T13:30:16Z
dc.date.copyright© 2024 Prudhomme et al.
dc.date.issued2024-12-23
dc.description.abstractLeishmaniasis is a disease caused by Leishmania parasites, transmitted by sand flies, and poses a major health risk in the Mediterranean region. Monitoring the spread of infected sand flies is crucial for controlling the disease. This study focused on improving the methods used to detect Leishmania in sand flies by comparing different DNA extraction techniques and assessing the accuracy of these methods across nine reference laboratories. All centers, except one, efficiently detected all Leishmania concentrations, demonstrating proficiency in diagnostic protocols. Moreover, we found that two specific DNA extraction kits, the EZ1 DSP Virus Kit and QIAamp DNA mini-kit, were the most effective for Leishmania detection. We also tested how sample preparation and shipping conditions affected the results, ensuring that our methods would work in real-world settings. Even under these conditions, the detection methods proved reliable. This work helps to standardize the detection of Leishmania, making surveillance more accurate and consistent. Continuous training and calibration are essential to ensure uniform diagnostic performance across laboratories, enhancing epidemiological surveillance and disease control strategies and enabling appropriate treatment.
dc.formatapplication/pdf
dc.format.extent15
dc.identifier.citationPLOS Neglected Tropical Diseases, 2024, Vol. 18, Issue 12 : e0012543
dc.identifier.doihttps://doi.org/10.1371/journal.pntd.0012543
dc.identifier.eissn1935-2735
dc.identifier.issn1935-2727
dc.identifier.urihttp://hdl.handle.net/10201/188290
dc.languageeng
dc.publisherPublic Library of Science
dc.relationThis study is catalogued by the CLIMOS Scientific Committee as CLIMOS number 011 (www.climos-project.eu).
dc.relation.publisherversionhttps://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0012543
dc.rightsAttribution 4.0 International
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titlePerformance evaluation of nine reference centers and comparison of DNA extraction protocols for effective surveillance of Leishmania-infected Phlebotomine sand flies: basis for technical recommendations
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
relation.isAuthorOfPublication8136045d-0c4c-468e-94c0-2f8dd71865fb
relation.isAuthorOfPublicationed7b26e8-ba82-4424-9d88-9146b063fb8c
relation.isAuthorOfPublication9c14eaee-8c65-40b7-af00-50f0a40d9191
relation.isAuthorOfPublication.latestForDiscovery8136045d-0c4c-468e-94c0-2f8dd71865fb
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