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Reliable and sensitive analytical platform to assess dietary exposure of pigs to mycotoxins and explore potential urinary biomarkers

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dc.contributor.authorCastell Martínez, Ana
dc.contributor.authorArroyo Manzanares, Natalia
dc.contributor.authorCampillo Seva, Natalia
dc.contributor.authorSanz Fernández, Santos
dc.contributor.authorRodríguez Estévez, Vicente
dc.contributor.authorRoquet, Josep
dc.contributor.authorGonzález, Antonio
dc.contributor.authorFenoll, José
dc.contributor.authorViñas López-Pelegrin, Pilar
dc.contributor.departmentQuímica Analítica
dc.date.accessioned2025-10-23T07:57:28Z
dc.date.available2025-10-23T07:57:28Z
dc.date.copyright© 2024 The Authors
dc.date.issued2024-12-24
dc.description.abstractA reliable and sensitive analytical platform is proposed for the assessment of pig exposure to mycotoxins through the consumption of commercial feed. A total of 48 naturally contaminated feed and 55 urine samples collected from eight Spanish farms were analyzed using a fast and simple methodology based on solid-liquid extraction (SLE) or liquid-liquid extraction (LLE) and dispersive liquid-liquid microextraction (DLLME). High-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) was used for the targeted analysis of 27 mycotoxins from different families in both matrices achieving limits of quantification in a range of 0.019–73.5 ng/g in feed and 0.011–31.7 ng/mL in urine. All feed samples showed contamination with at least 7 mycotoxins. Enniatins (A, A1, B and B1) and beauvericin were quantified in 100 % of feed samples. ENNB, tenuazonic acid (TeA) and deoxynivalenol (DON) were the mycotoxins with the highest mean total concentrations (1.0 ± 1.9 μg/g, 155 ± 209 ng/g and 81 ± 94 ng/g, respectively). In urine samples, DON, TeA, ENNB1 and ENNA were the most prevalent mycotoxins; and TeA, fumonisin B1 and alternariol had the highest mean total concentration (133 ± 199 ng/mg, 0.43 ± 1.3 μg/mg and 0.29 ± 1.3 μg/mg creatinine, respectively). Statistical tests revealed the correlation of DON and TeA occurrence in feed and urine. Untargeted analysis byHPLC coupled to quadrupole-time-of-flight mass spectrometer (Q-TOF-MS) yielded some urinary biomarkers of mycotoxin exposure and other relevant compounds such as certain antibiotic residues in urine.
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dc.format.extent10
dc.identifier.citationTalanta, 2025, Vol. 286 : 127441
dc.identifier.doihttps://doi.org/10.1016/j.talanta.2024.127441
dc.identifier.issn0039-9140
dc.identifier.urihttp://hdl.handle.net/10201/168409
dc.languageeng
dc.publisherElsevier
dc.relationMinisterio de Ciencia e Innovación (MCIN) (Proyecto PID2021-123201NB-I00)
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S003991402401823
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectTarget and untargeted analysis
dc.subjectMycotoxins
dc.subjectBiomarker
dc.subjectExposure
dc.subjectUrine
dc.subjectFeed
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleReliable and sensitive analytical platform to assess dietary exposure of pigs to mycotoxins and explore potential urinary biomarkers
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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relation.isAuthorOfPublication.latestForDiscovery1cbd3819-b58d-4f27-8714-d8941702c12e
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