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GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm

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dc.contributor.authorLlavanera, Marc
dc.contributor.authorDelgado-Bermúdez, Ariadna
dc.contributor.authorFernández-Fuertes, Beatriz
dc.contributor.authorRecuero, Sandra
dc.contributor.authorMateo, Yentel
dc.contributor.authorBonet, Sergi
dc.contributor.authorBarranco Cascales, Isabel
dc.contributor.authorYeste, Marc
dc.contributor.departmentMedicina y Cirugía Animal
dc.contributor.otherFacultad de Veterinaria
dc.date.accessioned2025-10-17T10:29:41Z
dc.date.available2025-10-17T10:29:41Z
dc.date.copyright© 2019 The Author(s)
dc.date.issued2019-08-05
dc.description.abstractBackground: Cryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination (AI) centres. However, it is well-known that cryopreservation procedures induce changes in the water phase in both intra and extracellular compartments, which alter the content and localisation of several proteins and ends up curtailing the structural integrity of functional sperm (i.e., cryoinjuries). Alterations and deficiencies of sperm-oocyte binding proteins during gamete recognition are one of the causes of reproductive failure both in vitro and in vivo. In this sense, characterisation of cryopreservation effects upon oocyte-binding proteins of sperm, such as IZUMO1 and GSTM3, is essential when assessing the impact of this technique in swine reproduction. Results: Cryopreservation was found to induce changes in the localisation of IZUMO1 and GSTM3 in boar sperm. However, the relative content of both proteins was not altered after thawing. Furthermore, whereas IZUMO1 content was found not to be related to the cryotolerance of boar sperm, GSTM3 content was observed to be higher in poor (PFE) than in good (GFE) freezability ejaculates in both pre-frozen (1.00 INT·mm2 ± 0.14 INT·mm2 vs. 0.72 INT·mm2 ± 0.15 INT·mm2; P < 0.05) and post-thawed (0.96 INT·mm2 ± 0.20 INT·mm2 vs. 70 INT·mm2 ± 0.19 INT·mm2; P < 0.05) samples. Moreover, GSTM3 levels were found to be higher in those spermatozoa that exhibited low mitochondrial activity, high reactive oxygen species (ROS) production, and high membrane lipid disorder post-thaw (P < 0.05). Conclusions: The difference in GSTM3 content between GFE and PFE, together with this protein having been found to be related to poor sperm quality post-thaw, suggests that it could be used as a cryotolerance marker of boar spermatozoa. Furthermore, both IZUMO1 and GSTM3 relocate during cryopreservation, which could contribute to the reduced fertilising capacity of frozen-thawed boar sperm.
dc.formatapplication/pdf
dc.format.extent11
dc.identifier.citationJournal of Animal Science and Biotechnology (2019) 10:61
dc.identifier.doihttps://doi.org/10.1186/s40104-019-0370-5
dc.identifier.eissn2049-1891
dc.identifier.urihttp://hdl.handle.net/10201/167391
dc.languageeng
dc.publisherBioMed Central
dc.relationThe authors would like to thank Estela Garcia and Technical Research Services of University of Girona for their technical support.The authors acknowledge the support from Ministry of Science, Innovation and Universities, Spain (Grants: RYC-2014-15581, AGL2017–88329-R and FJCI 2017-31689) and European Commission (H2020-MSCA-IF-79212).
dc.relation.publisherversionhttps://jasbsci.biomedcentral.com/articles/10.1186/s40104-019-0370-5
dc.rightsAttribution 4.0 Internacional
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBoar
dc.subjectCryopreservation
dc.subjectGSTM3
dc.subjectIZUMO1
dc.subjectROS
dc.subjectSperm
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleGSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
relation.isAuthorOfPublication3c34d0d5-7d7d-4bcc-a119-8906f53baa7b
relation.isAuthorOfPublication.latestForDiscovery3c34d0d5-7d7d-4bcc-a119-8906f53baa7b
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