Publication: The expression of a novel
cxcr4 gene in Xenopus embryo
Authors
Alonso, Edurne ; Gómez-Santos, Laura ; Sáez, F.J. ; Madrid Cuevas, Juan Francisco
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The aim of the present work was to identify a
homologue of zebrafish cxcr4b in Xenopus, which could
be involved in primordial germ cell (PGC) guidance
migration. Following a BLAST analysis, the clone gi
27519681, homologous to the zebrafish gene z-cxcr4b,
was identified, inserted into pCMV-SPORT6 plasmid
and cloned in Escherichia coli. Embryonic expression of
x-cxcr4b was analyzed by RT-PCR. X-cxcr4b was
weakly expressed maternally but sharply increased after
the mid-blastula transition (MBT), declining
significantly at stage 45 when PGCs migration is
complete. In contrast, RT-PCR of isolated presumptive
PGCs showed strong maternal expression at stage 8,
which decreased by stage 10 post-MBT and was not
detected at stage 14. Whole mount in situ hybridization
of x-cxcr4b mRNA showed that this gene is expressed in
neural and haematopoietic tissues, and should be linked
to important processes during embryonic development of
these organs. Although weak staining could be seen in
some samples within the anterior endoderm, expression
of x-cxcr4b was never coincident with that of Xpat
mRNA, which labels PGCs restricted to the posterior
endoderm. Therefore, maternal x-cxcr4b is specifically
downregulated within PGCs at pre-migratory stages
while it is expressed in other tissues.
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