Publication: Type II alveolar epithelial cells and free alveolar cells after intratumor TNF-a administration
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Date
1996
Authors
Sulkowska, M. ; Sulkowski, S. ; Nowak, H.F. ; Terlikowski, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The experiment used Morris hepatoma 5123
series growing in muscles of the Buffalo rats. A
suspension of 3 x 1 0 ~n eoplastic cells was injected into
the right hind leg of the animals. After fourteen days,
TNF-a was administered into the tumour in a dose of
1 .5x104 U124 hours in 0.5 m1 PBS solution. The group 1
animals were injected for 4 days and group 11 for 8 days.
Control groups consisted of rats with injected Morris
hepatoma which were given PBS solution instead of
TNF-a (group 111 A and B) and animals without the
hepatoma, given 4 or 8 TNF-a, respectively (groups IV
A and B). In the present study, we have explored the
effect of intratumor TNF-a administration on the
composition of cells isolated from the lungs through
multiple bronchoalveolar lavages (BAL). Ultrastructural
evaluation of the pulmonary tissue was done using a
transmission electron microscope (TEM), with special
attention paid to type 11 alveolar epithelial cells and free
alveolar cells. Examinations in TEM in groups 1, 11 and
IV (A and B) found, in the lumen of alveoli, an increase
in the number of alveolar macrophages (AM) with
morphological features of intensified activity and
AM with numerous secondary lysosomes containing
material of phospholipid structure. Also, numerous type
11 alveolar epithelial cells with emptied lamellar
bodies were observed. The above mentioned changes
were especially marked after eightfold TNF-a
administration.
In groups 1, 11 and IV (A and B), compared with group 111, a significant increase was found in the total
number of cells isolated by BAL as well as in the
number of cells with positive reaction in staining
according to Beckstead's method. It may indicate that
the changes in the parameters mentioned above are
related to TNF-a action. The results obtained indicate
the possibility of systemic effect of TNF-a after its
administration into the experimental Morris hepatoma.
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