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Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes.

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dc.contributor.authorLamey, Elsa
dc.contributor.authorRubio, Camila P
dc.contributor.authorCarreira, Laura
dc.contributor.authorCapela e Silva, Fernando
dc.contributor.authorMartinez Subiela, Silvia
dc.contributor.authorTecles, Fernando
dc.contributor.authorLopez Jornet, Pia
dc.contributor.authorCeron, Jose
dc.contributor.authorTvarijonaviciute, Asta
dc.contributor.departmentDermatología, Estomatología, Radiología y Medicina Física
dc.date.accessioned2024-01-29T19:20:26Z
dc.date.available2024-01-29T19:20:26Z
dc.date.issued2022-06-08
dc.description©2022. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/ This document is the Published, version of a Published Work that appeared in final form in Scientific Reports. To access the final edited and published work see 10.1038/s41598-022-13491-9es
dc.description.abstractThe present study aims to assess the effects of thermal and chemical inactivating procedures, that can be used for SARS-CoV-2 inactivation, on different salivary analytes. SDS–Polyacrylamide Gel Electrophoresis (SDS-PAGE) protein profile and a panel of 25 specific biomarkers of oxidative status, stress, metabolism and tissue damage were evaluated in samples subjected to different treatments: thermal (65 °C or 92 °C) and chemical with detergents [sodium dodecyl sulphate (SDS), Triton X-100 or NP-40]. Salivary SDS-PAGE profile was most affected by heating at 92 °C, with three and two protein bands decreasing and increasing their expression levels, respectively. This treatment also affected the results of several enzymes, with some of them being also affected by heating at 65 °C and incubation with SDS. The use of Triton X-100 or NP-40 resulted in increased values of cortisol, triglycerides and glucose, not affecting the other tested biomarkers. The present results will help researchers and clinicians to select the best protocols to work in safe conditions with saliva, taking into account the target analyte planned to be measured.es
dc.formatapplication/pdfes
dc.format.extent7es
dc.identifier.citationScientific Reports
dc.identifier.doi10.1038/s41598-022-13491-9
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10201/138028
dc.languageenges
dc.relationTis work is funded by National Funds through FCT-Foundation for Science and Technology under the Project UIDB/05183/2020es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectSARS‑COV‑2es
dc.subjectSalivaes
dc.titleEffect of thermal and chemical treatments used for SARS-COV-2 inactivation in the measurement of saliva analytes.es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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