Publication: Hu/elav RNA-binding protein HuR regulates parathyroid hormone related peptide
expression in human lung adenocarcinoma cells
Authors
Lauriola, Libero ; Serini, Simona ; Granone, Pierluigi ; Lanza, Paola ; Martini, Maurizio ; Calviello, Gabriella ; Oreste Ranelletti, Franco
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In 54 stage I and II human lung
adenocarcinomas, HuR and PTHrP levels were
positively correlated and the PTHrP-HuR status of the
tumor was an independent prognostic marker of the
clinical outcomes of patients. The possibility that HuR
could upregulate PTHrP expression in lung
adenocarcinoma was investigated by immunohistochemical,
Western blot and RT-PCR analyses in HCC44
and DV90 human lung adenocarcinoma cell lines. In
both cell lines, knockdown of HuR by specific siRNAs
reduced PTHrP mRNAs and both cellular and secreted
protein. Moreover, it inhibited cell growth and induced
cell apoptosis, as revealed by the increase of caspase-3
activity. These effects were partially rescued by the
addition of exogenous PTHrP (1-34). Analysis by
actinomycin D assay revealed that in both cell lines HuR
silencing produced a decrease of PTHrP mRNA half-life
by about 70%. These findings add PTHrP to the list of
lung cancer-associated genes, whose mRNA is stabilized
by HuR.
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Citation
Histology and Histopathology, vol. 28, nº 9 (2013)
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