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HM13 is a predictive biomarker of metastasis and neutrophil infiltration in colorectal cancer

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dc.contributor.authorYanbing Ren
dc.contributor.authorYing Mao
dc.contributor.authorXiao Yuan
dc.contributor.departmentBiología Celular e Histología
dc.coverage.spatial, , ,
dc.date.accessioned2025-10-13T11:12:15Z
dc.date.available2025-10-13T11:12:15Z
dc.date.issued2025
dc.description.abstractBackground. High levels of histo-compatibility minor 13 (HM13) have been related to the progression of several cancers. Here, we investigated the function of HM13 in colorectal cancer (CRC). Methods. HM13 expression, clinicopathology analysis, and its influence on survival were analyzed in multiple public databases (TCGA, TIMER2.0, and GEPIA). HM13 mRNA and protein levels in CRC cells were examined by qRT-PCR and western blot, respectively. CCK-8, Transwell, wound-healing, and adhesion assays were used to measure cell proliferation, migration, invasion, and adhesion in HM13-overexpressed and -silenced cells. The relationship between HM13 expression and neutrophil infiltration was also analyzed. CRC xenograft mouse models were used for in vivo verification of HM13 function. Results. In this study, TCGA dataset analysis revealed that elevated HM13 levels correlated with malignant progression and worse survival outcomes in CRC. Cell migration, proliferation, invasion, and adhesion were suppressed through the knockdown of sh-HM13 and enhanced through HM13 overexpression. Additionally, HM13 expression significantly correlated with the infiltration level of neutrophils in CRC in TCGA and TIMER analyses. HM13 levels were also positively correlated with myeloperoxidase (MPO) levels. In addition, in vivo studies further confirmed that MPO overexpression partially abolished the inhibition of tumor growth by sh-HM13 in CRC. Conclusion. The results suggested that high HM13 expression in CRC could promote tumor growth and metastasis by reducing neutrophil infiltration and may serve as a useful target in the treatment of metastatic CRC.
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dc.format.extent12
dc.identifier.citationHistology and Histopathology, Volúmen 40, nº 9 (2025)
dc.identifier.doihttps://doi.org/10.14670/HH-18-857
dc.identifier.eissn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/166149
dc.languageeng
dc.publisherUniversidad de Murcia, Histología e Histopatología
dc.relationSin financiación externa a la Universidad
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectMetastasis
dc.subjectNeutrophils
dc.subjectColorectal cancer
dc.subjectHM13
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleHM13 is a predictive biomarker of metastasis and neutrophil infiltration in colorectal cancer
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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Histology and histopathology Vol.40, nº9 (2025)