Publication: Immunohistochemical and lectin histochemical analysis of the alpaca efferent ducts
Authors
Parillo, F. ; Magi, G.E. ; Diverio, S. ; Catone, G.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
An immunohistochemical and lectin
histochemical study of the efferent ducts was performed
in the alpaca. Two types of epithelium, consisting of
principal and ciliated cells, were detected on the basis of
the different cytokeratins expression and lectin binding
pattern. AE1/AE3 and 13 cytokeratin antibodies
intensely immunostained the entire cytoplasm of type I
PCs, whereas AE1/AE3, but not anti cytokeratin 13,
immunoreacted in type II principal cells along the apical,
lateral and basal plasma-membrane.
The histochemical characterization of the epithelial
cells was carried out using a battery of different lectins:
Con-A, UEA-I, LTA, WGA, GSA-II, GSA-IB4, SBA,
PNA, ECA, DBA, MAL-II and SNA. Sialidase digestion
and deglycosilation pre-treatments were also employed.
In type I principal cells, the staining of the Golgi
zone was interpreted giving evidence for the synthesis
and secretion of O- and N-linked oligosaccharides. In
particular, a-Man/a-Glc, GlcNAc, ß-Gal-(1-4)-GlcNAc,
Neu5Aca2,3Gal and Neu5Aca2,6Gal/GalNAc residues
were included in both O- and N-linked glycans, whereas
a-Fuc, ß-GalNAc and a-Gal were only found in Olinked
oligosaccharides; a-GalNac and ß-Gal-(1-3)-DGalNAc
were found subterminal to sialic acid moieties
and they were included in O- and N-glycans. In type II
principal cells, the lectin staining was observed in the
apical cytoplasmic granules and in vacuoles that were
interpreted as components of an elaborate endocytotic
apparatus specialized for the uptake of particulate
material and fluid from the lumen. These results suggest
the existence of two structurally different epithelial
segments along the ductuli efferentes of the alpaca, with
a high degree of compartmentalization of the secretory
and absorptive activities.
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