Publication: Flavonoid profile of Lupinus mexicanus germinated
seed extract and evaluation of its neuroprotective effect
Authors
Uribe-Gómez, José de Jesús ; Zamora-Natera, Juan Francisco ; Bañuelos-Pineda, Jacinto ; Kachlicki, Piotr ; Stobieck, Maciej ; García-López, Pedro Macedonio
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
https://doi.org/10.14670/HH-29.1415
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info:eu-repo/semantics/article
Description
Abstract
The aim of this study was to determine the
flavonoid profile of Lupinus mexicanus germinated seed
extract (PE) and to evaluate its effect as a phytoestrogen
on the morphometric parameters of CA3 hippocampal
neurons of ovariectomized rats (OVX). L. mexicanus
seeds, germinated for 48 h, were homogenized and
macerated using an 80% ethanol solution. The extract
was analyzed by HPLC/MS-MS. Thirty young Wistar
strain female rats (200±10 g) were randomly distributed
into four groups: sham operated (S) treated with
dimethyl sulfoxide (vehicle); ovariectomized and treated
with 1250 µg of PE extract (OVX-PE); ovariectomized
and treated with 5 µg estradiol benzoate (OVX-EB); and
ovariectomized and vehicle treated (OVX). All
substances were injected subcutaneously daily for 28
days. On day 29, the animals were sacrificed, perfused,
and fixed to obtain the brains for histological processing.
Each brain was cut and stained with hematoxylin and
eosin. The thickness of the stratum oriens (SO), the
nuclear diameter, and the neuronal density were
measured in the hippocampus CA3 area. Nine different
flavonoids and one non-identified compound were
detected. The histological analysis demonstrated that the
thickness of the SO was higher in the OVX-EB and S
groups than in the OVX-PE and OVX groups (p<0.05);
in addition, the nuclear diameters of the neurons in the
OVX-EB and S groups were higher compared with the
other groups (p<0.05). The OVX group had the highest
cellular density among groups (p<0.05). Based on our
results, the PE obtained did not have beneficial effects
on CA3 hippocampal neurons.
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Citation
Histol Histopathol, Vol. 29, n.º 11 (2014)
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