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cAMP-Protein Kinase A and Stress-Activated MAP Kinase 1 signaling mediate transcriptional control of autophagy in fission yeast during glucose limitation or starvation.

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dc.contributor.authorPérez Díaz, Armando Jesús
dc.contributor.authorVázquez Marín, Beatriz
dc.contributor.authorVicente Soler, Jero
dc.contributor.authorPrieto Ruiz, Francisco
dc.contributor.authorSoto, Teresa
dc.contributor.authorFranco, Alejandro
dc.contributor.authorCansado Vizoso, José
dc.contributor.authorMadrid, Marisa
dc.contributor.departmentGenética y Microbiología
dc.date.accessioned2025-01-14T08:49:41Z
dc.date.available2025-01-14T08:49:41Z
dc.date.issued2022-09-26
dc.description© 2022 Informa UK Limited. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted Manuscript version of a Published Work that appeared in final form in Autophagy. To access the final edited and published work see https://doi.org/10.1080/15548627.2022.2125204
dc.description.abstractMacroautophagy/autophagy is an essential adaptive physiological response in eukaryotes induced during nutrient starvation, including glucose, the primary immediate carbon and energy source for most cells. Although the molecular mechanisms that induce autophagy during glucose starvation have been extensively explored in the budding yeast Saccharomyces cerevisiae, little is known about how this coping response is regulated in the evolutionary distant fission yeast Schizosaccharomyces pombe. Here, we show that S. pombe autophagy in response to glucose limitation relies on mitochondrial respiration and the electron transport chain (ETC), but, in contrast to S. cerevisiae, the AMP-activated protein kinase (AMPK) and DNA damage response pathway components do not modulate fission yeast autophagic flux under these conditions. In the presence of glucose, the cAMP-protein kinase A (PKA) signaling pathway constitutively represses S. pombe autophagy by downregulating the transcription factor Rst2, which promotes the expression of respiratory genes required for autophagy induction under limited glucose availability. Furthermore, the stress-activated protein kinase (SAPK) signaling pathway, and its central mitogen-activated protein kinase (MAPK) Sty1, positively modulate autophagy upon glucose limitation at the transcriptional level through its downstream effector Atf1 and by direct in vivo phosphorylation of Rst2 at S292. Thus, our data indicate that the signaling pathways that govern autophagy during glucose shortage or starvation have evolved differently in S. pombe and uncover the existence of sophisticated and multifaceted mechanisms that control this self-preservation and survival response.es
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dc.format.extent77es
dc.identifier.citationAutophagy, 2023, Vol. 19 (4), pp. 1311-1331
dc.identifier.doihttps://doi.org/10.1080/15548627.2022.2125204
dc.identifier.issnPrint: 1554-8627
dc.identifier.issnElectronic: 1554-8635
dc.identifier.urihttp://hdl.handle.net/10201/148383
dc.languageenges
dc.publisherTaylor and Francis Group
dc.relationÁmbito del proyecto: Nacional Agencia financiadora: Agencia Estatal de Investigación Convocatoria: Proyectos de I+D+i, modalidad "generación de conocimiento". Nombre del proyecto: Mecanismos de control de la citocinesis modulados por señales ambientales Código o número del acuerdo de subvención: PID2020-112569GB-I00es
dc.relation.publisherversionhttps://www.tandfonline.com/doi/full/10.1080/15548627.2022.2125204
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAutophagyes
dc.subjectcAMP protein kinase A
dc.subjectFermentation
dc.subjectGlucose
dc.subjectMAP kinase
dc.subjectRespiration
dc.subjectSchizosaccharomyces pombe
dc.subjectTranscription
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiologíaes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.titlecAMP-Protein Kinase A and Stress-Activated MAP Kinase 1 signaling mediate transcriptional control of autophagy in fission yeast during glucose limitation or starvation.es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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