Publication: Prognostic value of apoptosis in breast cancer (pT1-pT2). A TUNEL, p53,
bcl-2, bag-1 and Bax immunohistochemical study
Authors
Sirvent, J.J. ; Aguilar, M.C. ; Olona, M. ; Pelegrí, A. ; Blázquez, S. ; Gutiérrez, C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Apoptosis or programmed cell death
produces cells breaking into several fragments of nuclei,
cytoplasm or both nuclei and cytoplasm, known as
apoptotic bodies which can be visualized in
haematoxylin-eosin staining. Some genes (promoters
and suppressors) control this process and certain
mutations may induce the expression of abnormal
proteins, which can be detected by immunohistochemical
staining.
Apoptosis can be detected by the TUNEL method
either identifying apoptotic bodies or cells at the initial
stages of the fragmentation process.
We have studied 186 cases of infiltrating ductal
breast carcinoma, stages pT1-pT2, and analysed the
prognostic significance of tumour recurrence and overall
survival of apoptotic index (AI) through univariate and
multivariate analysis. We have also studied the
immunohistochemical protein expression of apoptosis
promoter and suppressors gene (p53, nuclear expression;
bcl-2 and Bax, cytoplasm expression; BAG-1, nuclear
and cytoplasm expression). The results indicate
prognostic significance of p53 and bcl-2 related to
patient death and bcl-2 and tumour size to tumour
recurrence, bcl-2 acting as a protector factor (apoptotic
suppressor) in both situations.
On the other hand, we have not found useful
prognostic information of AI either to tumour recurrence
or overall survival in univariate or multivariate studies. In this study, Bax expression does not provide a new
prognostic role in breast carcinoma, although it contrasts
to the bcl-2 action and accelerates death.
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