Publication: REG IV overexpression in an early stage of colorectal carcinogenesis, An immunohistochemical study
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Date
2010
Authors
Li, Xiao-Han ; Zheng, Yang ; Zheng, Hua-Chuan ; Takahashi, Hiroyuki ; Yang, Xiang-Hong ; Masuda, Shinji ; Takano, Yasuo
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
To clarify the role of REG IV, a new member
of the regenerating gene (REG) family, in tumorigenesis
and progression of colorectal carcinoma (CRC), 320
CRC specimens, 123 corresponding adjacent noncancerous
mucosa (ANCMs), 46 corresponding nonadjacent
non-cancerous mucosa (NANCMs) and 86
adenomas were investigated immunohistochemically to
compare REG IV expression with clinicopathological
features. In addition, double immunofluorescence
labeling was performed to analyze the localization of
REG IV and the intestinal mucin, MUC2. The
expression of REG IV in CRCs was significantly lower
than in NANCMs, ANCMs or adenomas, and inversely
correlated with poor differentiation and venous invasion.
In cases of ANCM, REG IV expression was positively
correlated with the depth of invasion, lymph node
metastasis and Duke’s staging of corresponding cases.
The expression of REG IV in CRC was significantly
linked to that of MUC2 and the EGFR phosphorylated
on Tyr1068, but not to that of MUC5AC, EGFR, Akt, or
Akt phosphorylated on Ser473 or Thr308. The double
immunofluorescence revealed coexpression, but
independent localization, of REG IV and MUC2 in
NANCMs, ANCMs, adenomas and CRCs, except for
mucinous carcinomas. Univariate analysis using the
Kaplan-Meier method indicated no correlation between
REG IV expression and the cumulative survival rate of
CRC patients. In conclusion, REG IV expression was
upregulated in ANCMs and adenomas, then decreased in
CRCs. This indicated that REG IV overexpression may
be an early event in CRC carcinogenesis. Its expression
in CRCs was positively linked to MUC2 and
phosphorylation of the EGFR on Tyr1068, suggesting that
REG IV may be a useful marker for intestinal type
mucinous carcinoma and a good candidate as a
molecular therapeutic target for CRCs.
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