Glycoconjugate expression on the cell wall of tps1/tps1 trehalose-deficient Candida albicans strain and implications for its interaction with macrophages

Vitse-Standaert, Annie; García-Peñarrubia, Pilar; Argüelles, Juan Carlos; Poulain, Daniel; Jouault, Thierry; Martínez-Esparza Alvargonzález, María Concepción; Tapia Abellán, Ana

Publication:
Glycoconjugate expression on the cell wall of tps1/tps1 trehalose-deficient Candida albicans strain and implications for its interaction with macrophages

dc.contributor.author	Vitse-Standaert, Annie
dc.contributor.author	García-Peñarrubia, Pilar
dc.contributor.author	Argüelles, Juan Carlos
dc.contributor.author	Poulain, Daniel
dc.contributor.author	Jouault, Thierry
dc.contributor.author	Martínez-Esparza Alvargonzález, María Concepción
dc.contributor.author	Tapia Abellán, Ana
dc.contributor.department	Bioquímica y Biología Molecular B e Inmunología
dc.date.accessioned	2024-07-02T08:34:04Z
dc.date.available	2024-07-02T08:34:04Z
dc.date.issued	2011-01-20
dc.description	© The Author 2011. This document is the Published version of a Published Work that appeared in final form in Glycobiology. To access the final edited and published work see https://doi.org/10.1093/glycob/cwr007
dc.description.abstract	The yeast Candida albicans has developed a variety of strategies to resist macrophage killing. In yeasts, accumulation of trehalose is one of the principal defense mechanisms under stress conditions. The gene-encoding trehalose-6-phosphate synthase (TPS1), which is responsible for trehalose synthesis, is induced in response to oxidative stress, as in phagolysosomes. Mutants unable to synthesize trehalose are sensitive to oxidative stress in vitro. In mice, the TPS1-deficient strain, tps1/tps1, displays a lower infection rate than its parental strain (CAI4). We have previously demonstrated the reduced binding capacity of tps1/tps1 and its lower resistance to macrophages. At the same time, its outer cell wall layer was seen to be altered. In this study, we show that depending on the culture conditions, the tps1/tps1 strain regulates the carbohydrate metabolism in a different way to CAI4, as reflected by the enhanced β-mannosylation of cell wall components, especially at the level of the 120 kDa glycoprotein species, accessible at the cell surface of tps1/tps1 when cultured in liquid medium, but not on solid medium. This leads to changes in its surface properties, as revealed by decreased hydrophobicity, and the lower levels of ERK1/2 phosphorylation and tumor necrosis factor-α (TNF-α) production in macrophages, thus increasing the resistance to these cells. In contrast, in solid medium, in which over-glycosylation was less evident, tps1/tps1 showed similar macrophage interaction properties to CAI4, but was less resistant to killing, confirming the protective role of trehalose. Thus, the lack of trehalose is compensated by an over-glycosylation of the cell wall components in the tps1/tps1 mutant, which reduces susceptibility to killing.	es
dc.format	application/pdf	es
dc.format.extent	10	es
dc.identifier.citation	Glycobiology 21(6):796-805, 2011
dc.identifier.doi	https://doi.org/10.1093/glycob/cwr007
dc.identifier.issn	Print: 0959-6658
dc.identifier.issn	Electronic: 1460-2423
dc.identifier.uri	http://hdl.handle.net/10201/142799
dc.language	eng	es
dc.publisher	Oxford University Press	es
dc.relation	This work was supported in part by the project BIO-BMC 06/01-0003 from DGI, Comunidad de Murcia, Spain. MM-E and AT-A were supported by the Fundación Séneca, Comunidad de Murcia, Spain.	es
dc.relation.publisherversion	https://academic.oup.com/glycob/article/21/6/796/1991071?login=true	es
dc.rights.accessRights	info:eu-repo/semantics/restrictedAccess
dc.subject	Candida albicans	es
dc.subject	Cell wall	es
dc.subject	Macrophages	es
dc.subject	Trehalose	es
dc.title	Glycoconjugate expression on the cell wall of tps1/tps1 trehalose-deficient Candida albicans strain and implications for its interaction with macrophages	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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