Publication:
Sperm proteome after interaction with reproductive fluids in porcine: from the ejaculation to the fertilization site

Simple item page
dc.contributor.authorLuongo, Chiara
dc.contributor.authorGónzalez-Brusi, Leopoldo
dc.contributor.authorGarcía-Vázquez, Francisco Alberto
dc.contributor.authorCots Rodríguez, Paula
dc.contributor.authorIzquierdo Rico, María José
dc.contributor.authorAvilés Sánchez, Manuel
dc.contributor.departmentBiología Celular e Histología
dc.date.accessioned2025-01-26T10:38:51Z
dc.date.available2025-01-26T10:38:51Z
dc.date.issued2020-08-22
dc.description© 2020 by the authors. This manuscript version is made available under the CC-BY- 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in International Journal of Molecular Sciences. To access the final edited and published work see https://doi.org/10.3390/ijms21176060
dc.description.abstractEjaculated sperm are exposed to different environments before encountering the oocyte. However, how the sperm proteome changes during this transit remains unsolved. This study aimed to identify proteomic changes in boar sperm after incubation with male (seminal plasma, SP) and/or female (uterine fluid, UF; and oviductal fluid, OF) reproductive fluids. The following experimental groups were analyzed: (1) SP: sperm + 20% SP; 2) UF: sperm + 20% UF; 3) OF: sperm + 20% OF; 4) SP + UF: sperm + 20% SP + 20% UF; and (5) SP+OF: sperm + 20% SP + 20% OF. The proteome analysis, performed by HPLC-MS/MS, allowed the identification of 265 proteins. A total of 69 proteins were detected in the UF, SP, and SP + UF groups, and 102 proteins in the OF, SP, and SP + OF groups. Our results showed a higher number of proteins when sperm were incubated with only one fluid than when they were co-incubated with two fluids. Additionally, the number of sperm-interacting proteins from the UF group was lower than the OF group. In conclusion, the interaction of sperm with reproductive fluids alters its proteome. The description of sperm-interacting proteins in porcine species after co-incubation with male and/or female reproductive fluids may be useful to understand sperm transport, selection, capacitation, or fertilization phenomena.es
dc.formatapplication/pdfes
dc.format.extent25es
dc.identifier.citationInt. J. Mol. Sci. 2020, 21, 6060
dc.identifier.doihttps://doi.org/10.3390/ijms21176060
dc.identifier.issnPrint: 1661-6596
dc.identifier.issnElectronic: 1422-0067
dc.identifier.urihttp://hdl.handle.net/10201/149292
dc.languageenges
dc.publisherMDPI
dc.relationThe research was supported by the Spanish Ministry of Science and Innovation (PID2019-106380RB-I00 and PGC2018-094781-B-100).es
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/21/17/6060
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPig
dc.subjectSpermatozoa
dc.subjectBiofluids
dc.subjectFertility
dc.subjectProteomics
dc.titleSperm proteome after interaction with reproductive fluids in porcine: from the ejaculation to the fertilization sitees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublicatione71795aa-9367-4602-b62d-f9909b2d3d73
relation.isAuthorOfPublicationb0205f82-a44c-47b6-8061-b55efb4e6717
relation.isAuthorOfPublicationeb2d72d3-a1d5-48cc-b71f-4aaf60a9aeac
relation.isAuthorOfPublication.latestForDiscoverye71795aa-9367-4602-b62d-f9909b2d3d73
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Luongo et al., 2020.pdf
Size:
4.72 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.26 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos