Publication: Characterization of rat testicular teratoma and
its derived cell lines, with particular reference
to possible mesenchymal differentiations
Authors
Yamate, Jyoji ; Gamou, Katsuhiro ; Izawa-Ogata, Keiko ; Kotera, Takashi ; Izawa, Takeshi ; Takenaka, Shigeo ; Sawamoto, Osamu ; Kuwamura, Mitsuru
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The original tumor, 4 cm in diameter, was
found in the left testis of a 2-month old SD rat. The
tumor consisted of well-differentiated, mature tissues
such as bone, cartilage, adipose tissue, smooth and
skeletal muscles, skin, hair, glands (salivary, sebaceous,
apocrine and pancreatic exocrine glands) and trachea, as
well as nerve tissues. The tumor was diagnosed as a
mature type of teratoma, a rare in rat testis. Cloned cell
lines (named TSD-B4S and TSD-F9R) were established
from the tumor; cellular properties of these cell lines
were similar to each other; basically, their cultured cells
exhibited vimentin-positive mesenchymal nature with
occasional cells reacting to α-smooth muscle actin, glial
fibrillary acidic protein and CD163 (a macrophage
marker). The cell lines showed tumorigenicity when
inoculated into nude mice, being composed of immature
mesenchymal cells arranged mainly in a sheet. In TSDB4S cells treated with differentiation factors, we
demonstrated mesenchymal differentiations towards
adipogenic, osteogenic and myofibrogenic cells. The cell
line (TSD-B4S) would become a useful tool for studies
on stem cell differentiation, because the teratoma arises
from primordial germ cells like embryonic stem cells.
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Citation
Histology and Histopathology, vol. 29, nº 9, (2014)
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