Changes of the salivary and serum proteome in canine hypothyroidism

González Arostegui, L. G.; Muñoz Prieto, Alberto; Rubio, C. P.; Cerón, J. J.; Bernal, L.; Rubić, I.; Mrljak, V.; González Sánchez, J. C.; Tvarijonaviciute, Asta

Publication:
Changes of the salivary and serum proteome in canine hypothyroidism

dc.contributor.author	González Arostegui, L. G.
dc.contributor.author	Muñoz Prieto, Alberto
dc.contributor.author	Rubio, C. P.
dc.contributor.author	Cerón, J. J.
dc.contributor.author	Bernal, L.
dc.contributor.author	Rubić, I.
dc.contributor.author	Mrljak, V.
dc.contributor.author	González Sánchez, J. C.
dc.contributor.author	Tvarijonaviciute, Asta
dc.contributor.department	Medicina y Cirugía Animal
dc.date.accessioned	2025-01-15T13:01:56Z
dc.date.available	2025-01-15T13:01:56Z
dc.date.issued	2024-01
dc.description	.© 2023 The Author(s). This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published, version of a Published Work that appeared in final form in Domestic Animal Endocrinology. To access the final edited and published work see https://doi.org/10.1016/j.domaniend.2023.106825	es
dc.description.abstract	In this study, changes in salivary and serum proteome of dogs with hypothyroidism were studied using tandem mass tags (TMT) labelling and liquid chromatography-mass spectrometry (LC-MS/MS). Saliva and serum proteome from 10 dogs with hypothyroidism were compared with 10 healthy dogs. In saliva, a total of seven proteins showed significant changes between the two groups, being six downregulated and one upregulated, meanwhile, in serum, a total of six proteins showed significant changes, being five downregulated and one upregulated. The altered proteins reflected metabolic and immunologic changes, as well as, skin and coagulation alterations, and these proteins were not affected by gender. One of the proteins that were downregulated in saliva, lactate dehydrognease (LDH), was measured by a spectrophotometric assay in saliva samples from 42 dogs with hypothyroidism, 42 dogs with non-thyroid diseases and 46 healthy dogs. The activity of LDH was lower in the saliva of hypothyroid dogs when compared to non-thyroid diseased dogs and healthy controls. This study indicates that canine hypothyroidism can produce changes in the proteome of saliva and serum. These two sample types showed different variations in their proteins reflecting physiopathological changes that occur in this disease, mainly related to the immune system, metabolism, skin and coagulation. In addition, some of the proteins identified in this study, specially LDH in saliva, should be further explored as potential biomarkers of canine hypothyroidism.	es
dc.format	application/pdf	es
dc.format.extent	9	es
dc.identifier.citation	Domestic Animal Endocrinology, 2024, Vol. 86 : 106825
dc.identifier.doi	https://doi.org/10.1016/j.domaniend.2023.106825
dc.identifier.issn	Print: 0739-7240
dc.identifier.issn	Electronic: 1879-0054
dc.identifier.uri	http://hdl.handle.net/10201/148494
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	L.G.G.-A. was funded by the Seneca Foundation (21453/FPI/20). A.M-P. (RYC2021-033660-I) and C.P.R. (RYC2021-034764-I) have a post-doctoral fellowship “Ramón y Cajal” supported by the Ministerio de Ciencia e Innovación, Agencia Estatal de Investigación (AEI), Spain, and The European Next Generation Funds (NextgenerationEU).	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S0739724023000413?via%3Dihub	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Biomarkers	es
dc.subject	Dogs	es
dc.subject	Hypothyroidism	es
dc.subject	Proteins	es
dc.subject	Porteomics	es
dc.subject	Saliva	es
dc.title	Changes of the salivary and serum proteome in canine hypothyroidism	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es