Publication: Loss of a reporter gene for green fluorescent protein during tumor progression suggests the recruitment of host cells in rats with experimentally induced colon cancer
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Date
2008
Authors
García-Olmo, Dolores C. ; Samos, Julia ; Picazo, María G.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The interactions between a host’s normal
cells and tumor cells appear to be of significant
importance during the development of tumors. In the
present study, we examined this issue using a cancer
model in vivo in which tumor cells were tagged with a
reporter gene for green fluorescent protein (GFP). We
used a model of colon cancer in immunocompetent rats,
which were given a subcutaneous injection of tumor
cells that had been transfected with a gene for GFP. We
found that the number of fluorescent cells decreased
with the progression of the primary tumors and that
lymph node and lung metastases were never
macroscopically fluorescent. No GFP-encoding
sequences were detected by PCR in many of the longterm
primary tumors, in most lymph node metastases
(86%) and in all lung metastases, whereas the detection
of mutated k-ras, which identified such cells as tumor
cells, was always positive. To explain these findings, we
present a brief review of the literature and postulate that
tumor growth did not occur exclusively as a result of the
division of the injected cells, but also involved
recruitment of host cells.
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