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Neuroinflammation and gliosis in the injured and contralateral retinas after unilateral optic nerve crush

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dc.contributor.authorCabrera Maqueda, José María
dc.contributor.authorBoia, Raquel
dc.contributor.authorLucas Ruiz, Fernando
dc.contributor.authorGonzález Riquelme, María José
dc.contributor.authorAmbrosio, Antonio Francisco
dc.contributor.authorSantiago, Ana Raquel
dc.contributor.authorVidal Sanz, Manuel
dc.contributor.authorAgudo Barriuso, Marta
dc.contributor.authorGalindo Romero, Caridad
dc.contributor.departmentOftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
dc.date.accessioned2024-02-07T12:18:52Z
dc.date.available2024-02-07T12:18:52Z
dc.date.issued2023-08-22
dc.description©2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted, version of a Published Work that appeared in final form in Experimental Eye Research. To access the final edited and published work see https://doi.org/10.1016/j.exer.2023.109627
dc.description.abstractThe main purpose of this study is to analyze the effects of unilateral optic nerve crush in the gene expression of pro- and anti-inflammatory mediators, and gliosis markers in injured and contralateral retinas. Retinas from intact, unilaterally optic nerve injured or sham-operated C57BL/6J mice were analyzed 1, 3, 9 and 30 days after the surgery (n = 5/group and time point) and the relative expression of TGF-β1, IL-1β, TNF-α, Iba1, AQP4, GFAP, MHCII, and TSPO was analyzed in injured and contralateral using qPCR. The results indicated that compared with intact retinas, sham-operated animals showed an early (day 1) upregulation of IL-1β, TNF-α and TSPO and a late (day 30) upregulation of TNF-α. In sham-contralateral retinas, TNF-α and TSPO mRNA expression were upregulated and day 30 while GFAP, Iba1, AQP4 and MHCII downregulated at day 9. Compared with sham-operated animals, in retinas affected by optic nerve crush GFAP and TSPO upregulated at day 1 and TNF-α, Iba1, AQP4 and MHCII at day 3. In the crushed-contralateral retinas, TGF-β1, TNF-α, Iba1 and MHCII were upregulated at day 1. TSPO was upregulated up to day 30 whereas TGF-β1 and Iba1 downregulated after day 9. In conclusion, both sham surgery and optic nerve crush changed the profile of inflammatory and gliosis markers in the injured and contralateral retinas, changes that were more pronounced for optic nerve crush when compared to sham.es
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dc.identifier.citationExperimental Eye Research, 235 (2023): 109627
dc.identifier.doihttps://doi.org/10.1016/j.exer.2023.109627
dc.identifier.issnPrint: 0014-4835
dc.identifier.urihttp://hdl.handle.net/10201/138882
dc.languageenges
dc.publisherElsevier
dc.relationInstituto de Salud Carlos III, Fondo Europeo de Desarrollo Regional “Una manera de hacer Europa” (PI19/00071); Spanish Ministry of Economy and Competitiveness (PID2019-106498GB-I00); and the Foundation for Science and Technology (FCT), Portugal (UIDB/04539/2020, UIDP/04539/2020).es
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectOftalmologíaes
dc.subjectNeurocienciaes
dc.subjectInflamaciónes
dc.subjectRetina
dc.subjectOptic nerve crush
dc.subjectInflammation
dc.subjectCytokines
dc.subjectGliosis
dc.subjectMicroglial cells
dc.subjectBilateral response
dc.titleNeuroinflammation and gliosis in the injured and contralateral retinas after unilateral optic nerve crushes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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