Publication: Analysis of the in vivo dend ritic
cell response to the bacterial superantigen
staphylococcal enterotoxin B in the mouse spleen
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Date
2001
Authors
Yoon, S. ; Bae, K. L. ; Shin, J. Y. ; Yoo, H. J. ; Lee, H. W. ; Baek, S. Y. ; Kim, B. S. ; Kim, J. B. ; Lee, H. D.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
To inv esti ga te th e in vi vo effec ts of
St<l ph ylococca l enterotoxin B (SEB) on dendritic ce lls
(DCs) in the splee n, a single dose of SEB (50 pg/kg)
was administered to BALB/c mice by intraperi to nea l
injection. Aft erwa rds, the mice were sacrificed at 2, 6
and 24 hr, 2, 4, 7 and 15 days, and the splee ns were
removed. The immunocytochemica l characterizati on of
th e ce lls was ca rri ed out usin g va ri ous monocl onal
anti bodi es in cryostat-cut sect ions.
The distributi on patt erns of DCs and th eir ma jor
costimulatory molecul es, CD80, CD86 and CD40 in the
spleen were identified, and the ev idence fo r maturation
of DCs ill vivo in response to SEB was obtained. It was
found that systemic administration of SEB induced the
migration of most of the immature, splenic DCs from the
marginal zone to the periarterial lymphatic sheath wjthin
6 hr. This movement paralleled a maturation process, as
assessed by upregul ati on of CD40, CD80 and CD86
ex pression in the interdigitating dendritic cells (IDCs).
The upregul ation of costimul atory molecul e expression
was co nspi c uous o nl y in DCs in co ntrast to oth e r
antige n- prese nting ce lls (APCs) such as mac rophages
and B ce lls w hi c h did no t s how a ny sig nifica nt
altera tions in their costimulatory molecul e expression.
We also demonstrated the temporal expression pattern of
these costimulatory molecul es on the activated DCs. The
upregul ation of costimulatory molecul es on DCs reached
a peak leve l 6 hr aft er SEB injection, while the increase
in number of T ce lls ex pressing T ce ll receptor Vf38
reached a peak level on day 2 after SEB treatment.
In co nclusion, we demonstr ated th e ill vivo DC
response to SEB in the mouse spleen, especiall y a potent
stimul ati ve effect of SEB on DCs in vivo, a temporal
distribution pattern of DCs as well as T cells incl uding TCR Vf38+ T ce lls, and a differenti al expression pattern
of costimul atory molecules on the acti va ted DCs. The
results of the present study indicate that DCs are the
principal type of APCs which mediate T cell activation
by SAg ill vivo, and that each costimulatory molecule
may have diffe rent role in the activation of DCs by SAg.
Thus, it is plausible to speculate that DCs playa critical
role in the T cell clonal expansion by SAgs and other
SAg-induced immune responses ill vivo.
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Citation
Histology and histopathology, Vol. 16, nº4 (2001)
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