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Dilute-and-shoot-liquid chromatography-quadrupole time of flight-mass spectrometry for pteridine profiling in human urine and its association with different pathologies

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dc.contributor.authorGiménez Campillo, Claudia
dc.contributor.authorPastor Belda, Marta
dc.contributor.authorArroyo Manzanares, Natalia
dc.contributor.authorCampillo Seva, Natalia
dc.contributor.authorVal Oliver, Blanca del
dc.contributor.authorZarauz García, José
dc.contributor.authorSáenz, Luis
dc.contributor.authorViñas López-Pelegrin, Pilar
dc.contributor.departmentQuímica Analítica
dc.date.accessioned2025-10-16T10:28:58Z
dc.date.available2025-10-16T10:28:58Z
dc.date.copyright© 2023 by the authors
dc.date.issued2023-06-01
dc.description.abstractPteridines are a group of compounds synthesised by many living organisms that are involved in the metabolism of many cofactors and vitamins. Their concentration in biological fluids may be altered by various pathologies such as cancer or inflammatory bowel disease, urine being the main route of excretion. In this study, three lumazines and ten pterins were analysed in their native oxidation state using high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. Their high concentration in urine samples and their good ionisation behaviour allow the use of the dilute-and-shoot method by simple filtration of the urine prior to chromatographic analysis. The final method offers excellent linearity, sensitivity and precision parameters, and a total of 135 urine samples were analysed from patients with some relevant information such as faecal calprotectin (FCP) levels, common diseases such as diabetes, hypertension and dyslipidaemia and immunological diseases such as inflammatory bowel disease (IBD). The pteridine profile was related to FCP levels without showing any correlation. In addition, pteridine levels were compared between healthy subjects and IBD, diabetic, hypertensive and dyslipidaemic patients, and significant differences were found between the two groups for some of the pteridines.
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dc.format.extent13
dc.identifier.citationChemosensors, 2023, Vol. 11 : 324
dc.identifier.doihttps://doi.org/10.3390/chemosensors11060324
dc.identifier.eissn2227-9040
dc.identifier.urihttp://hdl.handle.net/10201/166929
dc.languageeng
dc.publisherMDPI
dc.relationSpanish MCIN (Project PID2021-123201NB-I00 financed by MCIN/AEI/10.13039/501100011033/FEDER, UE)
dc.relation.publisherversionhttps://www.mdpi.com/2227-9040/11/6/324
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPteridine profile
dc.subjectUrine samples
dc.subjectDilute and shoot
dc.subjectHigh performance liquid chromatography
dc.subjectQuadrupole time of flight mass spectrometry
dc.subject.odsObjetivo 3: Salud
dc.titleDilute-and-shoot-liquid chromatography-quadrupole time of flight-mass spectrometry for pteridine profiling in human urine and its association with different pathologies
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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relation.isAuthorOfPublication.latestForDiscovery7f90d175-499a-4f4e-9848-7be5f431f7b3
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