Publication:
Rapid microglial activation induced by traumatic brain injury is independent of blood brain barrier disruption

Thumbnail Image
Files
Rapid microglial activation induced by traumatic brain injury is independent of blood brain barrier disruption.pdf(3.36 MB)
Date
2007
relationships.isAuthorOfPublication
relationships.isSecondaryAuthorOf
relationships.isDirectorOf
Authors
Koshinaga, M. ; Suma, T. ; Fukushima, M. ; Tsuboi, I. ; Aizawa, S. ; Katayama, Y.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Following CNS injury, microglia respond and transform into reactive species exhibiting characteristic morphological changes that have been termed “activated” or “ameboid” microglia. In an attempt to establish that microglial reactions induced immediately after injury are caused by intrinsic mechanisms rather than infiltration of blood and its constituents, oxygenized Ringer’s solution was perfused into the cerebral circulation of rats so that the circulating blood could be eliminated prior to injury induction. Under artificial respiration, a catheter was inserted from the cardiac apex into the ascending aorta, and oxygenized Ringer’s solution was immediately perfused with a pulsatile blood pump, resulting in wash out of the circulating blood from the brain within 1 min. Subsequently, a cortical contusion was induced in the unilateral parietal cortex using a controlled cortical impact (CCI) device. At 5 min following the injury, the brain was fixed by perfusion of fixative through the catheter and removed. Coronal vibratome sections were then processed for CR3 immunohistochemistry to examine the microglial activation. It appeared that microglial activation with both morphological transformation and an increase in CR3 immunoreactivity was induced throughout the hemisphere ipsilateral to the injury side exclusively, even in rats with elimination of circulating blood. The microglial reactions did not differ substantially from those observed in the control rats with extensive BBB disruption. The present results thus provide direct evidence that the microglial activation induced immediately after injury is independent of infiltration of circulating blood induced by concurrent BBB disruption.
publication.page.subject
Microglia , Brain injury
Citation
URI
http://hdl.handle.net/10201/27544
item.page.embargo
Collections
Histology and histopathology Vol.22, nº 2 (2007)
Ir a Estadísticas