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Impact of a shift work-like lighting schedule on the functioning of the circadian system in the short-lived fish Nothobranchius furzeri

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dc.contributor.authorAlmaida Pagán, Pedro Francisco
dc.contributor.authorOrtega-Sabater, C.
dc.contributor.authorLucas-Sánchez, A.
dc.contributor.authorMartinez-Nicolas, A.
dc.contributor.authorEspinosa, C.
dc.contributor.authorMadrid, J. A.
dc.contributor.authorRol, M.
dc.contributor.authorMendiola, P.
dc.contributor.authorCosta, J. de
dc.contributor.authorEsteban Abad, María de los Ángeles
dc.contributor.departmentFisiología
dc.date.accessioned2024-06-17T11:18:03Z
dc.date.available2024-06-17T11:18:03Z
dc.date.issued2018-09-02
dc.description© 2018 Elsevier Inc. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Experimental Gerontology. To access the final edited and published work see https://doi.org/10.1016/j.exger.2018.08.010
dc.description.abstractAdult Nothobranchius furzeri of the MZM-04/10 strain were individually kept and subjected to a “5+2” shifting lighting schedule (SHIFT) for 8 weeks in order to evaluate the desynchronizing effects of a simulated human-like shift-work schedule on the functioning of the circadian system (CS). With this aim, sixteen 21-week-old N. furzeri were placed into a Morning, Night and Evening schedule (lights on from 08:00 to 16:00, 00:00 to 08:00 and 16:00 to 00:00 h, respectively) and fed once a day in the middle of the corresponding photophase (12:00, 04:00 and 20:00 h, respectively). Then, in the weekends (2 days), fish were always returned to the Morning shift. As controls, 16 fish were maintained under a non-shifting LD cycle condition (CONTROL) throughout the whole experiment, with lights on from 08:00 to 16:00 h. Rest-activity rhythm (RAR) of fish subjected to SHIFT showed several symptoms of chronodisruption, such as a decrease in the percentage of diurnal activity and a reduction of the relative amplitude and the circadian function index with time. When a periodogram analysis was performed, RAR of N. furzeri under SHIFT conditions showed up to three separate circadian components: one longer than 24 h (26.5 h) that followed the weekly 8 h delays; a short-period component (~23 h) that was related to the weekend's phase advances, and finally, a 24 h component. The shifting LD schedule also affected fish CS at a molecular level, with several significant differences in the expression of core genes of the molecular clock (bmal1, clock, rorα, rev-erbα) between SHIFT and CONTROL animals. RAR impairment along with changes in clock gene expression could be associated with high stress and accelerated aging in these fish.es
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dc.identifier.citationExperimental Gerontology 112 (2018) 44–53
dc.identifier.doihttps://doi.org/10.1016/j.exger.2018.08.010
dc.identifier.issnPrint: 0531-5565
dc.identifier.urihttp://hdl.handle.net/10201/142356
dc.languageenges
dc.publisherElsevieres
dc.relationP.F.A.-P. was funded by a research grant from the Fundación ONCE, as part of the “Oportunidad al Talento” Programme (co-financed by ESF). This work was supported by the Ministry of Economy and Competitiveness, the Instituto de Salud Carlos III through the RETICEF Network (The Aging and Frailty Cooperative Research Network, RD12/ 0043/0011), a CIBERFES grant (CB16/10/00239) and a grant 19899/ GERM/15 awarded to JAM (co-financed by FEDER).es
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/experimental-gerontology/vol/112/suppl/Ces
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCircadian rhythmes
dc.subjectChronodisruption
dc.subjectNothobranchius
dc.subjectShift-work
dc.subjectMolecular clock
dc.titleImpact of a shift work-like lighting schedule on the functioning of the circadian system in the short-lived fish Nothobranchius furzeries
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublicatione9f74fca-ae81-48b7-9b53-185f049b49ad
relation.isAuthorOfPublication.latestForDiscoverye9f74fca-ae81-48b7-9b53-185f049b49ad
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