Publication: X-irradiation reduces lesion scarring at the contusion site of adult rat spinal cord
Authors
Zhang, S.X. ; Geddes, J.W. ; Owens, J.L. ; Holmberg, E.G.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Spinal cord injury (SCI) results in cell death
and tissue destruction, and ultimately cavitation
followed by the formation of lesion scars at the injury
site. The lesion scars include an astrocytic component
(glial scar) and a fibroblastic component (connective
tissue scar). The purpose of the present study is to
determine if X-irradiation could minimize the formation
of lesion scars and reduce the levels of chondroitin
sulfate proteoglycans (CSPGs) in the contusion SCI
model of the adult rat. Two weeks after SCI, a
connective tissue scar formed at the injury site consisting
primarily of fibroblasts and exhibits strong CSPG
immunoreactivity. The fibroblasts might originate from
the connective tissue of pia mater or arachnoid mater. At
the same time, reactive astrocytes in the spared tissue
accumulate surrounding the lesion cavity to form a thick
glial scar with significant enhancement of glial fibrillary
acidic protein (GFAP) and CSPG immunoreactivity.
After X-irradiation (40 Gy) of the injury site 2 days postinjury,
that results in an attenuated dose to the lesion, the
connective tissue scar was not observed, and
accordingly, almost no CSPG immunoreactivity was
detected at this area. Meanwhile, the glial scar and its
CSPG immunoreactivity were prominently reduced. Xirradiation
did not show significant improvement in
locomotor recovery, but resulted in a slight delay of body weight recovery following injury. This preparative
treatment could be used to reduce secondary scarring in
the lesion resulting in an enriched site for further
treatment such as growth related transplantation.
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