Publication: Morphological effects of electrical stimulation and intermittent muscle stretch after immobilization in soleus muscle
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Date
2006
Authors
Mattiello-Sverzut, A.C. ; Carvalho, L.C. ; Cornachione, A. ; Nagashima, M. ; Neder, L. ; Shimano, A.C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The objective of the present study was to
assess the effectiveness of a combined protocol of
muscle stretching and strengthening after immobilization
of the hindlimb. Thirty female Wistar rats were divided
into 6 groups: group immobilized for 14 days to cause
full plantar flexion by cast (GI, n=6); group
immobilized/stretched (GIS, n=6): submitted to the same
immobilization and to 10 days of passive stretching;
group immobilized/electrically stimulated (GIES, n=6):
similarly immobilized and submitted to 10 days of low
frequency electrical stimulation (ES); group
immobilized/stretched/electrically stimulated (GISES,
n=6): similarly immobilized, submitted to 10 days of
stretching and ES application; group immobilized/free
(GIF, n=3): similarly immobilized and then left with free
limbs for 10 days; control group (CG, n=3). The middle
portion of the soleus muscle was frozen and sections
were stained with HE or mATPase. Morphological
analysis revealed high cellular reactivity in the GISES,
GIES and GIS groups. The lesser diameter and
proportion of type I fibers (TIF) and type II fibers (TIIF)
(at pH 9.4) and connective area (at HE stain) were
measured with an image analyzer and the data obtained
were analyzed statistically by the unpaired Student t-test
(p² 0.05). The results indicated that: a) immobilization
generated atrophy of both fiber types (p<0.05); b) joint
application of ES and stretching was not efficient in reestablishing the size of the two fiber types compared to
CG (p<0.05); c) the ES protocol reestablished only the
size of TIIF, which showed values similar to those
detected in CG (p<0.05); d) the stretch increased the
proliferation of the perimysium connective tissue
(p>0,05). Thus, we conclude that, in the model applied
here to female rats, a stretching protocol may limit the volume protein gain of soleus muscle fibers and increase
the connective interstitial tissue.
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