Publication: Immunolocalization of matrix proteins in different human cartilage subtypes
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Date
2006
Authors
Wachsmuth, L. ; Söder, S. ; Fan, Z. ; Finger, F. ; Aigner, T.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Cartilage exerts many functions in different
tissues and parts of the body. Specific requirements
presumably also account for a specific biochemical
composition. In this study, we investigated the presence
and distribution pattern of matrix components, in
particular collagen types in the major human cartilages
(hyaline, fibrous, and elastic cartilage) by histochemical
and immunohistochemical means.
Macroscopically normal articular cartilages, menisci,
disci (lumbar spine), epiglottal, and tracheal tissues were
obtained from donors at autopsy. Aurical and nasal
cartilages were part of routine biopsy samples from
tumor resection specimens. Conventional histology and
immunohistochemical stainings with collagen types I, II,
III, IV, V, VI, and X and S-100 protein antibodies were
performed on paraformaldehyde-fixed and paraffinembedded
specimens.
The extracellular matrix is the functional component
of all cartilages as indicated by the low cell densities. In
particular major scaffold forming collagen types I (in
fibrous cartilage) and II (in hyaline and elastic
cartilages) as well as collagen type X (in the calcified
layer of articular cartilages, the inner part of tracheal
clips, and epiglottis cartilage) showed a specific
distribution. In contrast, the "minor" collagen types III,
V, and VI were found in all, collagen type IV in none of
the cartilage subtypes.
In this study, we present a biochemical profile of the
major cartilage types of the human body which is
important for understanding the physiology and the
pathophysiology of cartilages.
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