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Early cytomegalovirus reactivation in renal recipients is associated with high levels of b cell maturation antigen transcript expression prior to transplantation

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dc.contributor.authorAlfaro, Rafael
dc.contributor.authorRodríguez Aguilar, Luis
dc.contributor.authorLlorente, Santiago
dc.contributor.authorJiménez Coll, Víctor
dc.contributor.authorMartínez Banaclocha, Helios
dc.contributor.authorGalián, José Antonio
dc.contributor.authorBotella, Carmen
dc.contributor.authorMoya Quiles, María Rosa
dc.contributor.authorMuro Pérez, Manuel
dc.contributor.authorMinguela, Alfredo
dc.contributor.authorMuro, Manuel
dc.contributor.authorLegaz Pérez, Isabel
dc.contributor.departmentCiencias Sociosanitarias
dc.date.accessioned2024-07-08T11:46:31Z
dc.date.available2024-07-08T11:46:31Z
dc.date.issued2023-06-22
dc.description© 2023 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in International Journal of Molecular Sciences. To access the final edited and published work see https://doi.org/10.3390/ijms241310491
dc.description.abstractCytomegalovirus (CMV) infection is the most frequent infection episode in kidney transplant (KT) recipients. Reactivation usually occurs in the first three months after transplantation and is associated with higher cellular and/or antibody-mediated rejection rates and poorer graft performance. CMV induces the expression of BAFF (B-cell-activating factor, a cytokine involved in the homeostasis of B cells), which communicates signals for survival and growth to B cells and virus-specific plasma cells via the R-BAFF (BAFF receptor), TACI (the calcium modulator, the cyclophilin ligand interactor), and BCMA (B cell maturation antigen) receptors. These molecules of the BAFF system have also been suggested as biomarkers for the development of alloantibodies and graft dysfunction. This prospective study included 30 CMV-IgG seropositive KT recipients. The expression levels of the genes BAFF-R, transmembrane activator and CAML interactor (TACI), and B cell maturation antigen (BCMA) in peripheral blood leukocytes (PBL) pre-KT were determined using qPCR. qPCR was also used to monitor CMV reactivation in the first three months following KT. The remainder of the KT recipients were classified as CMV− reactivation, and those with more than 500 copies/mL in at least one sample were classified as CMV+ reactivation. There were no discernible variations in the BAFF-R and TACI transcript expression levels. In the CMV+ group, we examined the relationship between the transcript levels and peak viremia. Peak viremia levels and BCMA transcript levels showed a strong correlation. BAFF-R and TACI expressions showed no measurable differences. In patients with early CMV reactivation, high BCMA receptor expression was associated with increased plasmablast, lymphocyte B cell class-switched levels (LBCS), and viral load. Our findings demonstrate that pre-KT BCMA transcript levels increased in KT recipients with early CMV reactivation. These transcript levels positively correlate with peak viremia and weakly with plasmablast and LBCS levels in PBLs.es
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dc.format.extent13es
dc.identifier.citationInt. J. Mol. Sci. 2023, 24(13), 10491
dc.identifier.doihttps://doi.org/10.3390/ijms241310491
dc.identifier.issnPrint: 1661-6596
dc.identifier.issnElectronic: 1422-0067
dc.identifier.urihttp://hdl.handle.net/10201/142914
dc.languageenges
dc.publisherMDPI
dc.relationOur work was possible thanks to support from Instituto de Salud Carlos III (ISCIII), Spanish Ministry of Economy and Competitiveness. Grant Number P19/01194 and co-funding of the European Union with European Fund of Regional Development (FEDER) with the principle of “A manner to build Europe”es
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/24/13/10491
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBCMAes
dc.subjectCMVes
dc.subjectBAFF gene expressiones
dc.subjectAcute rejectiones
dc.subjectKidney transplantes
dc.subjectPlasmablastes
dc.titleEarly cytomegalovirus reactivation in renal recipients is associated with high levels of b cell maturation antigen transcript expression prior to transplantationes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublicationb83b4b59-2d61-40f0-9108-5c6a6d158295
relation.isAuthorOfPublication.latestForDiscoveryb83b4b59-2d61-40f0-9108-5c6a6d158295
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