Publication: Levels of activity of superoxide dismutase in seminal plasma do not predict fertility of pig AI-semen doses
Authors
Barranco, Isabel ; Padilla, Lorena ; Tvarijonaviciute, Asta ; Parrilla, Inmaculada ; Martinez, Emilio A. ; Rodríguez Martínez, Heriberto ; Yeste, Marc ; Roca, Jordi
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DOI
https://doi.org/10.1016/j.theriogenology.2019.08.010
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info:eu-repo/semantics/article
Description
©2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Accepted, version of a Published Work that appeared in final form in Theriogenology. To access the final edited and published work see https://doi.org/10.1016/j.theriogenology.2019.08.010
Abstract
Superoxide dismutase (SOD) is a major antioxidant enzyme in boar seminal plasma (SP). This study evaluated how SP-SOD affected sperm attributes when semen of boars of various breeds, included in commercial artificial insemination (AI)-programs, was extended and liquid-stored at 17 °C for AI; as well as their in vivo fertility (farrowing rate and litter size of 10,952 AI-sows). SP-SOD-activity was assessed in 311 ejaculates (100 boars) while sperm motility (by CASA), viability and intracellular H2O2 generation in viable spermatozoa (by flow cytometry) were measured at 0 and 72 h of liquid storage. SP-SOD activity was not affected by breed but differed (P < 0.001) between boars (n = 50), ranging from 1.16 ± 0.11 to 7.02 ± 0.75 IU/mL. Semen AI-doses (n = 44) hierarchically grouped (P < 0.001) with low SP-SOD activity showed lower (P < 0.05) sperm motility and intracellular H2O2 at 72 h of liquid storage. Fertility did not differ between AI-boars (n = 39) hierarchically grouped (P < 0.001) with high or low SP-SOD activity. In conclusion, SP-SOD activity is boar dependent and positively related with sperm functionality of liquid-stored semen AI-doses. However, this positive effect is not reflected on in vivo fertility post-AI.
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Citation
Theriogenology (2019) 140:18-24.
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