Publication: Induction of sesquiterpenes, phytoesterols and extracellular pathogenesis-related proteins in elicited cell cultures of Capsicum annuum
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Date
2010-07-01
Authors
Ferrer Ayala, María Angeles ; Ros Barceló, Alfonso ; Almagro Romero, Lorena ; Belchí Navarro, Sarai ; Pedreño García, María Ángeles ; Sabater Jara, Ana Belén
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.jplph.2010.04.015
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Description
© 2010 Elsevier GmbH. This document is the Published Manuscript version of a Published Work that appeared in final form in Journal of Plant Physiology. To access the final edited and published work see https://doi.org/10.1016/j.jplph.2010.04.015
Abstract
Capsicum annuum suspension cell cultures were used to evaluate the effect of cyclodextrins and methyl
jasmonate as elicitors of defence responses. The induced defence responses included the accumulation
of sesquiterpenes and phytosterols and the activation of pathogenesis-related proteins, leading to reinforcement and modification of the cell wall architecture during elicitation and protection cells against
biotic stress. The results showed that the addition of both cyclodextrins and methyl jasmonate induced
the biosynthesis of two sesquiterpenes, aromadendrene and solavetivone. This response was clearly synergistic since the increase in the levels of these compounds was much greater in the presence of both
elicitors than when they were used separately. The biosynthesis of phytosterols was also induced in the
combined treatment, as the result of an additive effect. Likewise, the exogenous application of methyl
jasmonate induced the accumulation of pathogenesis-related proteins. The analysis of the extracellular
proteome showed the presence of amino acid sequences homologous to PR1 and 4, NtPRp27-like proteins
and class I chitinases, peroxidases and the hydrolytic enzymes LEXYL1 and 2, arabinosidases, pectinases,
nectarin IV and leucin-rich repeat protein, which suggests that methyl jasmonate plays a role in mediating defence-related gene product expression in C. annuum. Apart from these methyl jamonate-induced
proteins, other PR proteins were found in both the control and elicited cell cultures of C. annuum. These
included class IV chitinases, -1,3-glucanases, thaumatin-like proteins and peroxidases, suggesting that
their expression is mainly constitutive since they are involved in growth, development and defence
processes.
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Citation
Journal of Plant Physiology, 2010, Vol. 167, Issue 15, pp. 1273-1281
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